Dr.
Narendra K. Singh, Professor
Molecular
biology of plants and fungi
Office:
326 Life Sciences Building
Laboratory: 334 Life Sciences Building
Phone: (334) 844-1667/ 1645 (FAX)
E-mail: singhna@auburn.edu
Teaching
I have taught following courses at
● Principles of
Biology (BIOL 1020)
● Plant Gene Expression (BIOL 5320/6320)
● Introductory Molecular Genetics (BIOL 5220/6220)
● Recombinant DNA and Gene Expression (BIOL 5521/6521)
● Undergraduate
seminars (BIOL 4950): Protein sorting, Transgenics, SiRNA, Central dogma
exceptions
● Special
topics (BIOL 7970): Stress proteins, Molecular biology of plant development
● Undergraduate research (BIOL 4980)
● Special problems (BIOL 7980)
Research
interests
● Cellular
responses to environmental stress: gene
expression and metabolic changes in Arabidopsis mutants, cultured plant
cells and fungi
● Edible
vaccines: development of plant derived
edible vaccines against poultry viral diseases like IBDV and ARV
● Genetic manipulation of higher fungi
Selected
Publications
Selected publications representing area
of research interests
· Singh,
N.K., P.C. LaRosa, P.M. Hasegawa, A.K.
Handa and R.A. Bressan. 1986. Hormonal regulation
of protein synthesis associated with NaCl adaptation in plant cells. Proc.
Natl. Acad. Sci. U.S.A. 84:739-743.
· Singh,
N.K., C.A. Bracker, P.M. Hasegawa, A.K.
Handa, M.A. Hermodson, E. Pfankoch, F.E. Regnier and R.A. Bressan. 1987.
Characterization of osmotin: A thaumatin like protein associated with osmotic adaptation in plant
cells. Plant Physiol. 85:529-536
· Singh,
N.K., D.E. Nelson, D.N. Kuhn, P.M.
Hasegawa and R.A. Bressan. 1989.
Molecular cloning of osmotin and regulation of its expression by
· Narsimhan,
M.C., M.L. Binzel, E. Perez-Prat, Z. Chen, D.E. Nelson, N.K.
Singh, R.A.
Bressan and P.M. Hasegawa. 1991. NaCl
regulation of the gene encoding the 70 kDa subunit of tonoplast ATPase in
tobacco cells. Plant Physiol. 97:562-568.
· Kononowicz,
A.K., D.E. Nelson, N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1992.
Regulation of the Osmotin gene promoter.
Plant Cell 4:513-524.
· Nelson,
D.E., K.G. Raghothama, N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1992.
Analysis of structure and transcriptional activation of the osmotin
gene. Plant Molec. Biol. 19:577-588.
· Peng,
M., P.A. Lemke and N.K. Singh 1993.
Nucleotide sequence involved in replicative transformation of a
filamentous fungus. Curr. Genet.
24:114-121.
· Smeda,
R., P.M. Hasegawa, P.B. Goldsborough, N.K.Singh and S.C. Weller. 1993.
A serine to thrionine substitution in triazine herbicide-binding protein
in potato cells results in atrazine resistance without impairing productivity.
Plant Physiol. 103:911-917.
· Herzog,
R.W., N.K.
Singh, C.
Schmidt and P.A. Lemke. 1995. Presence of a P1 bacteriophage sequence in
transforming plasmids of Pleurotus
ostreatus. Current Genetics. 27:460-465.
· Redkar,
R.J., R.D. Locy and N.K. Singh. 1995. Biosynthetic pathways of glycerol
accumulation under salt stress in Aspergillus
nidulans. Experimental Mycology. 19:241-246.
· Redkar,
R.J., P.A. Lemke and N.K. Singh. 1996. Isolation of differentially expressed cDNA
clones from salt- adapted Aspergillus
nidulans. Current Genetics. 29:130-135.
· Locy,
R.D., C.C. Chang, B.L. Nielsen and N.K. Singh. 1996. Photosynthesis in salt-adapted heterotrophic
tobacco cells and regenerated plants. Plant Physiol. 110:321-328.
· Abed,
L.R., D. Liu, M.P. Durzo, M.L. Narsimhan, N.K. Singh, P.M. Hasegawa and. R.A. Bressan.
1996. The antifungal activity of osmotin involves the fungal plasma membrane
and cell wall. Plant Science. 118:11-23.
· Chang,
P.F., B. Damsz, A.K. Kononowicz, A. Chen, Yi Xu, N.K. Singh, M.L. Narshimhan, P.M. Hasegawa, and R.A. Bressan. 1996.
Alteration in cell membrane associated protein after adaptation to osmotic
stress. Physiol. Plant. 98:505-516.
· Redkar,
R.J., R.W. Herzog and N.K. Singh. 1998. Transcriptional activation of the Aspergillus nidulans gpd promoter
by osmotic signals. Applied Env.
Microbiol. 64:2229-2234.
· Wu,
S-J., R.D. Locy, J.J. Shaw, J.H. Cherry and N.K. Singh. 2000. Mutation in Arabidopsis
HIT1 locus causing heat and osmotic hypersensitivity. J. Plant Physiol.
157:543-548.
· Wang,
S-Y, S-J. Wu, G. Thottappilly, R.D. Locy, and N.K. Singh. 2001. Molecular cloning and
structural analysis of the gene encoding Bacillus cereus exochitinase
Chi36. JBB 92:59-66.
· Narsimhan,
M.L., H. Lee, B. Damsz, N.K. Singh, J.I. Ibeas, T.K. Mastmuto, C.P. Woloshuk, and R.A.
Bressan.2003. Overexpression of a cell wall glycoprotein in Fusarium oxysporum increases virulence
and resistance to a plant PR-5 protein. Plant Jour. 36: 390-400.
·
· Wu,
H-Z, N.K.
Singh, R.D.
Locy, K.S. Gunn, and J.J. Giambrone. 2004. Expression of immunogenic VP2 of
IBDV in transgenic Arabidopsis thaliana.
Biotechnology letters 26: 787-792.
· Wu,
H-Z., K.S. Gunn, N.K. Singh, R.D. Locy, and J.J. Giambrone. 2005. Yeast derived
sigma C protein induced immunity against avian reovirus. Avian Disease
49:281-285.
Graduate students
Present affiliations of students mentored as
major professor at Auburn University
● Dr. Ching-Chun Chang, MS (1994): Assistant Professor, Department of Botany,
● Dr. Rajendra J. Redkar, Ph.D. (1995): Group Leader-Molecular Biology, Schott-Glass
Inc.
● Dr. Roland W. Herzog, Ph.D. (1996): Associate Professor, Pediatric Gene Therapy,
● Dr. Sagadevan M. Mundree, Ph.D. (1996): Associate Professor, Molecular Biology,
● Dr. Shu-Yi Wang, Ph.D. (2000):
● Ms. Quing Luo, MS (2002): Present position not known
● Ms.
My current laboratory group
includes
 |
 |
 |
| Ms. Chia Chen Weng, Lab Tech | Ms. Yuying Sang, Ph.D. student | Ms. Nisreen Al-Qurran, Ph.D. student |