Mass Spectrometry


Mass Spectrometry Center


You can submit your samples for analysis via our online submittal form.

MSC provides collaborative assistance to PIs in the collection of preliminary data, preparation of proposals, and publication of manuscripts. Our contributions involve consultation, data analysis, and the preparation and review of manuscripts. We provide letters of support to PIs that describe the instrumentation and capabilities of the MSC.


Types of analysis we provide include:

Small Molecules

  • LC-MS and LC-MS/MS of small molecules with a quadrupole orbitrap (Orbitrap Exploris 120) and electrospray (ESI) or atmospheric chemical ionization (APCI)
  • Chromatography with Vanquish Binary UPLC, UV Detection, and various columns
    • Compound Discoverer software
    • Perfluorinated alkyl substance compatible
  • GC-MS with our Waters GCT Premier (Time-of-Flight) or Agilent 5975 MSD (single quadrupole)
    • MassLynx, MZmine, MetaboAnalyst, Enhanced ChemStation, AMDIS software

Large Molecules

  • Large molecules, especially proteins, can be analyzed with our Orbitrap Exploris 240 and EasySpray nanoflow U3000 liquid chromatography system
    • Whole protein analysis with Electrospray ionization (ESI) and calculation of the protein mass through deconvolution
    • Digestion with protease(s) and separation via LC and MS/MS to determine peptide sequence, usually leading to protein identity with MASCOT, Sequest, and Proteome Discoverer Software (also known as protein sequencing, shotgun proteomics, or bottoms-up)

 

lcms_orbitrap_exploris_120.jpg                 lcms_orbitrap_exploris_240.jpg
     
LC/MS: Thermo Scientific Vanquish UHPLC and Orbitrap Exploris 120 Mass Spectrometer   LC/MS: Thermo Scientific UltiMate 3000 UHPLC and Orbitrap Exploris 240 Mass Spectrometer
     
     
gcms_waters_gct_premier.jpg   agilent_5975_msd.jpg
     
 GC/MS: Waters GCT Premier orthogonal acceleration time-of-flight mass spectrometer   GC/MSD: Agilent gas chromatograph with 5975 mass selective detector

 

We have an instrument on display in the hallway of the Chemistry Building.
Click MALDI-TOF Display for more information. 

 

 

Mass Spectrometry Center
172 Chemistry Building

Melissa Boersma
AUmspec@auburn.edu
(334) 844-6911
 
Rates for federally funded projects are $48 per hour.
Rates will be increasing to $52 per hour, effective December 1, 2021.