Mass Spectrometry


Mass Spectrometry Center

You can submit your samples for analysis via our online submittal form at  https://aub.ie/MS_submittal

MSC provides collaborative assistance to PIs in collection of preliminary data, preparation of proposals and publication of manuscripts. Our contributions involve consultation, data analysis, and the preparation and review of manuscripts. We provide letters of support to PIs that describe the instrumentation and capabilities of the MSC.

Types of Analysis we provide include:

  • Small molecules  
    • LC-MS and LC-MS/MS of small molecules with a quadrupole orbitrap (Orbitrap Exploris 120) and electrospray (ESI) or atmospheric chemical ionization (APCI)
    • Chromatography with Vanquish Binary UPLC, UV Detection, and various columns
      • Compound Discoverer software
      • Perflourinated alkyl substance compatible
    • GC-MS with our Waters GCT Premier (Time-of-Flight) or Agilent 5975 MSD (single quadrupole)
      • MassLynx, MZmine, MetaboAnalyst, Enhanced ChemStation, AMDIS software
  • Large molecules especially proteins, with our Orbitrap Exploris 240 and EasySpray nanoflow U3000 liquid chromatography system
    • Whole protein analysis with Electrospray ionization (ESI) and calculation of the protein mass through deconvolution
    • Digestion with protease(s) and separation via LC and MS/MS to determine peptide sequence usually leading to protein identity with MASCOT, Sequest, and Proteome Discoverer Software (also known as protein sequencing, shotgun proteomics, or bottoms-up)

LC and MS systems at AU.jpg

LC/MS Thermo Fisher Scientific Orbitrap Exploris 240 and 120 with U3000 and Vanquish LC systems

 

GCT Premier

GC/MS Waters GCT Premier

We have an instrument in the hallway of the chemistry building.  Click MALDI-TOF Display for more information. 

 

 

 

Mass Spectrometry Center
172 Chemistry Building

Melissa Boersma
AUmspec@auburn.edu
(334) 844-6911
 
Rates for federally funded projects are $48 per hour. Rates will be increasing to $52 per hour effective December 1, 2021.