development of multiplex realtime biosensors for detection of pathogens* 

As clearly demonstrated by the several anthrax attacks, frequent outbreaks of foodborne pathogens, and increase of nosocomial infections in the United States, it is imperative for “point of care” facilities to possess rapid and accurate pathogen detection systems to minimize the damage to human health. As a member of the Auburn University Detection and Food Safety (AUDFS) group, my laboratory has been involved in developing accurate and user-friendly biosensors for rapid (less than 15 minutes including sample preparation) and realtime detection of pathogens.  Our biosensors are composed of phage-displayed oligopeptide probes for molecular recognition and magnetostrictive particles as sensor platforms. Specifically, my group has focused on improving the phage-display technology for biosensors and isolation and characterization of oligopeptide probes for pathogen capture.  We recently developed a novel method to immobilize phage displayed probes on the sensor platforms to improve the biosensor performance. We have also recently isolated and characterized phage-displayed oligopeptide probes for use in magnetoelastic particle based biosensors for realtime detection of various serovars of pathogenic Salmonella enterica, the leading causative agent of foodborne illness. Our current goal is to convert our singleplex biosensors into a multiplex platform for simultaneous realtime detection of multiple important bacterial pathogens. 

*This is a collaborative study with Dr. Bryan Chin, the Director of AUDFS and a professor of Materials Engineering.

  Sang-Jin Suh © Auburn University 2012. All rights reserved