MATHEWS LAB
Diabetes/Obesity Research
home
Protocols
Acid and Base preparation
cDNA synthesis
Cell culture etiquette
Chemiluminescence - UVP BioChemi Imager
Common Reagents
Glucose uptake in L6-GLUT4-myc cells
Glycogen synthesis in L6-GLUT4-myc cells
iCycler Real-time PCR
Immunoprecipitation
Lab Basics
Lipofectamine 2000 transfection
Primer Dilutions
Protein assay using BioRad reagent
RNA isolation from tissues
SDS-PAGE (using NOVEX pre-cast gels)
Western blotting
