Dr. Cornelius Varnado

Dr. Varnado was one of the Original Three graduate students to join the Goodwin laboratory.  Neil hails from McComb, Mississippi.  He received his Bachelor of Science degree in Chemistry from Alcorn State University where he was honored as an Alcorn State University Academic Scholar.  In his time with the Department of Chemistry and Biochemistry here at Auburn University, Neil was awarded as a Southern Regional Education Board Doctoral Fellow through the Alabama Commission on Higher Education.  While in the Goodwin laboratory, Neil developed a system to assist us in the expression of our catalase-peroxidases.  He and others have also been able to apply the system to the expression of other recombinant hemoproteins.  Neil also reported the characterization of a catalase-peroxidase from E. coli O157:H7.  This enzyme is part of a unique subgroup of catalase-peroxidases that are distinguished by their periplasmic location.  Interestingly, a significant proportion of these enzymes are carried by highly virulent bacterial pathogens and are thought to be virulence factors.  His work has also focused on a unique set of post-translational modifications that occur among the catalase-peroxidases.  In June 2006, Neil defended his doctoral dissertation entitled: Enhancing Expression of Recombinant Hemoproteins: Progress Towards Understanding Structure/Function and Therapeutic Application. Dr. Varnado has gone on to Rice University to do postdoctoral research with Professor John Olson at Rice University.

Publications and Abstracts

Kudalkar, S.N., Campbell, R.A., Li, Y., Varnado, C.L., Prescott, C., and Goodwin D.C.  2012.  Enhancing the peroxidatic activity of KatG by deletion mutagenesis" J. Inorg. Biochem. 116, 106 - 115.

Varnado, C. L., and Goodwin D. C.  2004.  Heme- and peroxide-dependent formation of a novel crosslink in catalase-peroxidases.  Amer. Chem. Soc. Southeast Regional Meeting, Research Triangle Park, NC.

Varnado, C. L., and Goodwin, D. C.  2004.  System for the expression of recombinant hemoproteins in Escherichia coli. Protein Expr. Purif. 35, 76 - 83.

Varnado, C. L., Hertwig, K. M., Thomas, R., Roberts, J. K., and Goodwin, D. C. 2004.  Properties of a novel periplasmic catalase-peroxidase from Escherichia coli O157:H7. Arch. Biochem. Biophys. 421, 166 - 174.

Goodwin, D. C., Baker, R. D., Varnado, C. L., and Li, Y.  2003.  Structural requirements for the hemoprotein-dependent decomposition of hydroperoxides:  lessons from the catalase-peroxidases.   Amer. Chem. Soc. Southeast Regional Meeting, Atlanta, GA.

Varnado, C. L., Hertwig, K. M., Thomas, R., Roberts, J. K., and Goodwin, D. C.  2003.  Characterization of a novel periplasmic catalase-peroxidase.  Amer. Chem. Soc. Southeast Regional Meeting, Atlanta, GA.

Varnado, C. L., Hertwig, K. M., Thomas, R., Roberts, J. K., and Goodwin, D. C.  2003.  Spectral and kinetic properties of a novel periplasmic catalase-peroxidase.  Amer. Chem. Soc. 226th National Meeting, New York, NY.

Varnado, C. L., and Goodwin, D. C. 2002.  Design of an expression system for recombinant hemoproteins.  Amer. Chem. Soc. Southeast Regional Meeting, Charleston, SC.