Rene is the third student to join the Goodwin
laboratory from the Republic of Cameroon. He began his studies at
Auburn University in the Fall of 2013, and joined the lab in
January 2014. Rene is working on a new research project for
our group. In collaboration with Dr. Angela Calderon in the
Harrison School of Pharmacy, we are evaluating shikimate kinase
from Mycobacterium tuberculosis (MtSK) as a target
for the development of new leads for antitubercular agents. Our
laboratory has developed a construct for the expression of MtSK.
Rene is using site-directed mutagenesis to produce informative
variants to investigate mechanisms of catalysis and as well as
mechanisms of inhibition by candidate inhibitors identified from
marine natural products. The goal is to produce a panel of MtSK
variants which will facilitate high throughput screening of
Publications and Abstracts
M. S., Fuanta, N. R., Jarrard, M. A., Hobrath, J. V., Goodwin,
D. C., Rants'o, T. A., and Calderon, A. I. 2017. A multifaceted
approach to identify non-specific enzyme inhibition: Application
to Mycobacterium tuberculosis shikimate kinase. Bioorg.
Medicin. Chem. Lett. (in press).
N. R., Simithy, J., Gill, G., Kollhoff, A., Childers, T.,
Calderon, A. I., and Goodwin D. 2016. Towards high-throughput
drug screening and mechanistic evaluation of tuberculosis
shikimate kinase; Intrinsic protein fluorescence. National Organization for
the Professional Advancement of Black Chemist and Chemical
Engineers (NOBCChE), Raleigh, NC (11/16
R., Simithy, J., Gill, G., Kollhoff, A., Childers, T., Calderon,
A. I., and Goodwin D. 2016. Targeted intrinsic protein
fluorescence, an approach towards high-throughput drug screening
and mechanistic evaluation of tuberculosis shikimate kinase. Southeast Meeting of
American Chemical Society (SERMACS), Columbia, SC (10/16).
M. S., Jarrad, M. A., Fuanta, N. R., Goodwin, D. C., and
Calderon, A. I. 2016. LC-MS based approach to characterize
non-specific binding inhibitors to Mycobacterium
tuberculosis shikimate kinase (MtSK). 64th
American Society for Mass Spectrometry National Conference on
Mass Spectrometry and Allied Topics, San Antonio, TX. (6/5/16
McCarty, S.E.; Schellenberger, A.; Goodwin, D.C.; Fuanta, N.R.;
Tekwani, B.L.; Calderon, A.I. 2015. Plasmodium
falciparum thioredoxin reductase (PfTrxR) and its role as a
target for new antimalarial discovery. Molecules 20, 11459
Kudalkar, S. N., Njuma, O. J., Li, Y., Muldowney, M., Fuanta, N.
R., and Goodwin, D. C. 2015. A role for catalase-peroxidase
large loop 2 revealed by deletion mutagenesis: Control of active
site water and ferric enzyme reactivity. Biochemistry 54,
1648 - 1662.
Fuanta, N. R., Simithy, J., Gill, G., Kollhoff, A., Childers, T.,
Calderon, A. I., and Goodwin D. 2015. Site-directed incorporation
of intrinsic fluorescence in shikimate kinase to evaluate
catalysis and inhibition. 6th Annual Southeast Enzymes Conference,
Georgia State University, Atlanta, GA (4/11/15).