Rene is the third student to join the Goodwin
laboratory from the Republic of Cameroon. He began his studies at
Auburn University in the Fall of 2013, and joined the lab in
January 2014. Rene is working on a new research project for
our group. In collaboration with Dr.
Angela Calderon in the Harrison School of Pharmacy, we are
evaluating shikimate kinase from Mycobacterium tuberculosis (MtSK)
as a target for the development of new leads for antitubercular
agents. Our laboratory has developed a construct for the
expression of MtSK. Rene is using site-directed
mutagenesis to produce informative variants to investigate
mechanisms of catalysis and as well as mechanisms of inhibition by
candidate inhibitors identified from marine natural products. The
goal is to produce a panel of MtSK variants which will facilitate
high throughput screening of candidate inhibitors.
Publications and Abstracts
McCarty, S.E.; Schellenberger, A.; Goodwin, D.C.; Fuanta, N.R.;
Tekwani, B.L.; Calderón, A.I. 2015. Plasmodium falciparum
thioredoxin reductase (PfTrxR) and its role as a target
for new antimalarial discovery. Molecules 20,
11459 - 11473.
Kudalkar, S. N., Njuma, O. J., Li, Y., Muldowney, M., Fuanta, N.
R., and Goodwin, D. C. 2015. A role for
catalase-peroxidase large loop 2 revealed by deletion
mutagenesis: Control of active site water and ferric enzyme
reactivity. Biochemistry 54, 1648 - 1662.
Fuanta, R., Simithy, J., Gill, G., Kollhoff, A., Childers, T.,
Calderon, A. I., and Goodwin D. 2015. Site-directed
incorporation of intrinsic fluorescence in shikimate kinase to
evaluate catalysis and inhibition. 6th Annual Southeast Enzymes
Conference, Georgia State University, Atlanta, GA (4/11/15).