Olive joined the Goodwin laboratory in the
Summer of 2012. She came to us by something of a circuitous
route, having first worked in the laboratory of an Organic
Division faculty member. When that individual took a position at
another university, Olive decided to remain at Auburn and switch
her emphasis to biochemistry. We are delighted to have her.
Olive has focused her efforts upon understanding the mechanisms by
which the catalase activity of KatG is inactivated, and how
peroxidatic electron donors prevent this process, and therefore,
stimulate catalase activity. These phenomena are
particularly prominent at lower pH (e.g., 5.0), and this is
especially important because host responses to pathogen attack
often involve production of copious quantities of hydrogen
peroxide and are carried out under acidic pH conditions.
Stimulation of KatG catalase activity by peroxidatic electron
donors may have a role in the virulence of several pathogens which
rely on KatG as an antioxidant defensive mechanism. Perhaps,
no pathogen in this group is more prominent than Mycobacterium
tuberculosis. KatG is the only enzyme this organism
produces that has catalase activity, and it is known that the
greatest abundance of M. tuberculosis cells in infected
human hosts are found inside neutrophils. Olive has applied
site-directed mutagenesis, steady-state kinetic, as well as
transient-state kinetic methodology to investigate KatG catalase
inactivation and rescue by peroxidatic electron donors. Indeed,
she is the first from our laboratory to make extensive use of
rapid-freeze-quench EPR to investigate KatG protein-based
radicals. In this effort, we have collaborated with the laboratory
of Dr. Aimin Liu now at University of Texas at San Antonio, most
especially Ian Davis, a graduate student in the group. Olive also
recently traveled to the National High Magnetic Field Laboratory
in Tallahassee, FL to perform high frequency EPR measurements on
some of her freeze-quenched samples. On June 2, 2016, Olive
successfully defended her dissertation Resolving the
Paradoxical Nature of a Bifunctional Enzyme: Pathways and
Regulation of Intramolecular Electron Transfer in KatG.
Olive engaged in postdoctoral research in the laboratory of
Professor Fred Guengerich at the Vanderbilt University School of
Medicine before moving on to California, first to Molecular
Assemblies, Inc. She is now in Torrance, CA, working with
PolyPeptide Group as a Senior Analytical Development Scientist.
Publications and Abstracts
Njuma, O. J., Davis, I.,
Ndontsa, E. N., Krewall, J. R., Liu, A., and Goodwin, D. C.
2017. Mutual synergy between catalase and peroxidase
activities of the bifunctional enzyme KatG is facilitated by
electron-hole hopping within the enzyme. J.
Biol. Chem. 292, 18408 - 18421.
Krewall,
J.
R., Njuma, O. J., and Goodwin, D. C. 2017. Role reversal
between peroxidase reaction intermediates generates the
distinct catalase mechanism of catalase-peroxidase. 46th Annual
Southeast Magnetic Resonance Conference, Tallahassee,
FL, (10/29/17).
Xu,
H.,
Krewall, J. R., Njuma, O. J., and Goodwin, D. C. 2017. How an
arginine switch preserves the catalase activity of KatG:
Strategic use of an active-site tryptophan for off-pathway
electron transfer. 46th
Annual Southeast Magnetic Resonance Conference,
Tallahassee, FL, (10/29/17).
Xu,
H., Krewall, J. R., Njuma, O. J., Davis, I., Liu, A., and
Goodwin, D. C. 2017. Using an arginine switch and an active
site tryptophan to direct off-pathway electron transfer:
Maximizing catalase activity from a peroxidase scaffold. Gordon Research
Conference: Enzymes, Coenzymes, and Metabolic Pathways,
Waterville Valley, NH, (7/16/17 – 7/21/17).
Krewall,
J.
R., Xu, H., Njuma, O. J., and Goodwin, D. C. 2017. Directing
off-pathway protein oxidation to preserve enzyme activity: At
last, a role for the proximal tryptophan of KatG. 8th Annual
Southeast Enzyme Conference, Atlanta, GA, (04/08/17).
Xu,
H.,
Krewall, J. R., Njuma, O. J., and Goodwin, D. C. 2017. How an
arginine switch preserves the catalase activity of KatG:
Strategic use of an active-site tryptophan for off-pathway
electron transfer. 8th
Annual Southeast Enzyme Conference, Atlanta, GA,
(04/08/17).
Kudalkar, S. N., Njuma, O. J., Li, Y., Muldowney, M., Fuanta,
N. R., and Goodwin, D. C. 2015. A role for
catalase-peroxidase large loop 2 revealed by deletion
mutagenesis: Control of active site water
and ferric enzyme reactivity. Biochemistry54,
1648 - 1662. Njuma, O. J., Davis, I.,
Ndontsa, E. N., Liu, A., and Goodwin, D. C. 2015. Proximal
tryptophan and arginine switch participation in
catalase-peroxidase inactivation. Gordon Research
Conference: Enzymes, Coenzymes, and Metabolic Pathways,
Waterville Valley, NH (7/13/15 -7/14/15) Njuma, O. J., Davis, I.,
Ndontsa, E. N., Liu, A., and Goodwin, D. C. 2015.
Participation of the proximal tryptophan as a potential
conduit for catalase-peroxidase inactivation. 6th Annual
Southeast Enzymes Conference, Georgia State University,
Atlanta, GA (4/11/15).
Njuma, O. J., Ndontsa, E. N., and Goodwin, D.C. 2015.
Electron donors to therescue: The proximal Trp as a potential
conduit for catalase-peroxidase inactivation.Departmental Seminar,Department of Chemistry,
University of Buea,
Cameroon. (4/1/15).
McCurdy, E., Barr, L.,
Njuma, O.J., Ndontsa, E. N., and Goodwin, D.C. 2015.
Evaluating the novel role of Trp 438 in active turnover of Mycobacterium
tuberculosis catalase-peroxidase. Auburn University J. of
Undergrad. Scholarship.4, 27 - 32.
Njuma,
O. J., Ndontsa, E. N., and Goodwin D. C. 2014. Catalase in
peroxidase clothing: Interdependent cooperation of two
cofactors in the catalytic versatility of KatG. Arch. Biochem. Biophys.
544, 27 – 39. Njuma,
O. J., Ndontsa, E.N., and Goodwin, D.C. 2014.
pH-dependent catalase stimulation of
catalase-peroxidases by peroxidatic electron donors.43rd
Southeastern Magnetic Resonance Conference (SEMRC), Tuscaloosa, AL. (10/25/14)
Njuma, O. J., Ndontsa, E.N., and Goodwin, D.C. 2014.
Evaluating an atypical mechanism of H2O2
decomposition by catalase-peroxidases. The Southeast Regional
Meeting of the American Chemical Society (SERMACS),
Nashville, TN. (10/18/14)
Njuma, O. J., Ndontsa, E. D., and Goodwin, D. C. 2014.
Evaluating the role of peroxidatic reducing substrates in an
unusual catalase activity of catalase-peroxidases. 2014 Symposium, The
Protein Society, San Diego, CA. (7/27/14)
Njuma,
O. J., Ndontsa, E. D., and Goodwin, D. C. 2014. Synergistic
effect of peroxidatic electron donors on the catalase activity
of Catalase-Peroxidase. 5th
Annual Southeast Enzymes Conference, Georgia State
University, Atlanta, GA. (4/5/14)
Njuma, O. J., Ndontsa, E. N. and Goodwin, D. C. 2014. Evaluating the role of electron donors in a novel
mechanism of H2O2 decomposition by
Catalase-Peroxidase. 91stAlabama Academy of Science Meeting (AAS), Auburn
University, AL (3/13/14) (Won first prize for
poster presentation).
McCurdy, E., Njuma, O. J., Ndontsa, E., and Goodwin, D. 2014.
W438 and the Diminished Necessity for Peroxidatic Rescue of
KatG Catalatic Turnover. 34th Annual
Undergraduate Research Conference, University of
Memphis, Memphis, TN.
McCurdy, E.,
Njuma, O. J., Ndontsa, E. N., and Goodwin, D. C. 2013. An
Investigation of W438 as a Potential Route for Off-Pathway
Electron Transfer and Its Relationship to the
Bifunctional Activity of Catalase-Peroxidase. Southeast
Regional Meeting of the American Chemical Society (SERMACS),
Atlanta, GA. (Won first prize for undergraduate poster
session). Njuma,
O. J., Ndontsa, E. N., and Goodwin, D.C. 2013. Rescue of
catalase-inactive intermediates of KatG by peroxidatic
electron donors. Southeast Regional Meeting of the
American Chemical Society (SERMACS), Atlanta, GA
Njuma, O. J.,
Ndontsa, E. N and Goodwin, D.C. 2013. KatG:
Improvisation of novel peroxide decomposition mechanisms. 99th
Annual Southeastern Branch of American Society
of Microbiology Meeting (SEBASM), Auburn
University, AL.
Njuma, O. J., Ndontsa, E.N., and Goodwin, D.C. 2013. KatG:
Bacterial improvisation of novel peroxide decomposition
mechanisms. Three
minute thesis, Auburn University, Auburn, AL.
Njuma, O. J., Ndontsa, E. N.,
and Goodwin, D. C. 2013. Electron donors to the rescue:
Evaluating a novel mechanism of hydrogen
peroxide decomposition by catalase-peroxidases. National
Meeting of the National Organization fortheProfessional Advancement of Black Chemists and Chemical Engineers (NOBCCHE),
Indianapolis,IN.
Njuma,
O. J., Ndontsa, E. N., and Goodwin, D.C. 2013. Surprising
role of peroxidatic electron donors in the catalase activity
of catalase-peroxidase.Diversity Awareness Symposium, Tuscaloosa,AL. (Award-winning poster)
Njuma, O. J., Ndontsa, E. N., and Goodwin, D.C. 2013. Surprising
role
of peroxidatic electron donors in the catalase activity of
Catalase-Peroxidase.Fourth Southeast Enzymes Conference,Atlanta, GA.