CURRICULUM VITA

 

Douglas C. Goodwin

Department of Chemistry and Biochemistry

Auburn University

Auburn, AL  36830

 

 

 

A.  Education

 

Ph.D., Biochemistry, Department of Chemistry and Biochemistry, Utah State University, August 1996, Thesis:  Redox Mediation in Peroxidase-Catalyzed Oxidation

 

B.A., Food, Nutrition, and Dietetics; Minor: Chemistry; University of Northern Colorado, December 1991, Magna Cum Laude

 

B.  Professional Experience

 

Associate Professor, Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, August 2005 – present

 

Assistant Professor, Department of Chemistry, Auburn University, Auburn, AL, September 1999 – July 2005

 

Research Associate, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, October 1996 - August 1999

 

C.  Honors, Awards, and Fellowships

 

Golden Key National Honor Society Teaching Award.  Golden Key Honor Society, Auburn University Chapter, April 2001.

 

Center in Molecular Toxicology Postdoctoral Research Trainee.  Department of Biochemistry, Vanderbilt University School of Medicine, July 1998 – August 1999.

 

Thomas F. Emery Memorial Research Scholar.  Department of Chemistry and Biochemistry, Utah State University, May 1995.

 

Willard L. Eccles Family Foundation Fellow.  College of Science, Utah State University, October 1992- September 1995.

 

George H. and Billie Bush Emert Scholar.  Department of Chemistry and Biochemistry, Utah State University, May 1996.

 

E.L. and Inez Waldron Travel Award.  Biotechnology Ctr, Utah State University, June 1996.

 

D.  Publications

 

26. Moore, R.L., Cook, C.O., Williams, R., and Goodwin, D. C.  2008.  Substitution of strictly conserved Y111 in catalase-peroxidase:  Impact of remote interdomain contacts on active site structure and catalytic performance.  J. Inorg. Biochem. 102, 1819 – 1824.

 

25. Cook, C.O., Moore, R.L., and Goodwin, D.C.  2008.  The effect of R117 and D597 interdomain residue substitutions on the reactivation of Escherichia coli catalase-peroxidase.  NOBCChE Proceedings 35, (in press).

 

24. Moore, R.L., Powell, L.J., and Goodwin, D. C.  2008.  The kinetic properties producing the perfunctory pH profiles of catalase-peroxidases. Biochim. Biophys. Acta. 1784, 900 – 907.

 

23. Trostchansky, A., O¹Donnell, V.B., Goodwin, D.C., Landino, L.M., Marnett, L.J., Radi, R., and Rubbo, H.  2007.  PGHS-1 in turnover is inactivated by peroxynitrite derived- radicals: Differential effect of ·NO on peroxidase and cyclooxygenase activities. Free Rad. Biol. Med. 41, 1029 – 1038.

 

22.  Baker, R. D., Cook, C. O., and Goodwin, D. C. 2006.  Catalase-peroxidase active site restructuring by a distant an inactive domain.  Biochemistry 45, 7113-7121.

 

21.  Goodwin, D. C., Laband, K. A., and Hertwig, K. M.  2005.  Capsaicinoid oxidation by peroxidases:  kinetic, structural, and physiological considerations. In  Phenolics in Foods and Natural Health Products.  Ho, C. T., and Shahidi, F., Eds. ACS Symposium Series, Vol. 909, pp. 161-174.

 

20.  Baker, R. D., Cook, C. O., and Goodwin, D. C.  2004.  Properties of catalase-peroxidase lacking its C-terminal domain.  Biochem. Biophys. Res. Comm. 320, 833-839.

 

19.  Li, Y., and Goodwin, D. C. 2004.  Vital roles of an interhelical insertion in catalase-peroxidase bifunctionality. Biochem. Biophys. Res. Comm.  318, 970-976.

 

18.  Varnado, C. L., and Goodwin, D. C.  2004.  System for the expression of recombinant hemoproteins in Escherichia coli. Prot. Exp. Purif.  35, 76-83.

 

17.  Varnado, C. L., Hertwig, K. M., Thomas, R., Roberts, J. K., and Goodwin, D. C. 2004.  Properties of a novel periplasmic catalase-peroxidase from Escherichia coli O157:H7.  Arch. Biochem. Biophys. 421, 166-174.

 

16.  Goodwin, D.C., and Hertwig, K. M. 2003.  Peroxidase-catalyzed oxidation of capsaicinoids:  Steady-state and transient-state kinetic studies.  Arch. Biochem. Biophys. 417, 18-26.

 

15.  Goodwin, D.C., Rowlinson, S. W., and Marnett, L. J.  2000.  Substitution of tyrosine for the proximal histidine ligand to the heme of prostaglandin endoperoxide synthase-2:  Implications for the mechanism of cyclooxygenase activation and catalysis.  Biochemistry 39, 5422-5432.

 

14.  Kiefer, J. R., Pawlitz, J. L., Moreland, K. T., Stegeman, R. A., Hood, W. F., Gierse, J. K.,  Stevens, A. M., Goodwin, D. C, Rowlinson, S. W., Marnett, L. J., Stallings, W. C., and Kurumbail, R. G.  2000.  Structural insights into the stereochemistry of the cyclooxygenase reaction.  Nature 405, 97-101.

 

13.  Rowlinson, S. W., Crews, B. C., Gierse, J. K., Goodwin, D. C. and Marnett, L. J. 2000.  Spatial requirements for 15-HETE synthesis within the cyclooxygenase active site of murine COX-2:  why acetylated COX-1 does not synthesize 15-R-HETE.  J. Biol. Chem. 275, 6586-6591.

 

12.  Goodwin, D. C., Landino, L. M., and Marnett, L. J.  1999.  Effects of nitric oxide and nitric oxide-derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis.  FASEB J. 13, 1121-1136.

 

11.  Goodwin, D. C., Landino, L. M., and Marnett, L. J.  1999.  Reactions of prostaglandin endoperoxide synthase with nitric oxide and peroxynitrite. Drug Metabolism Reviews 31, 273-294.

 

10.  Marnett, L. J., Rowlinson, S. W., Goodwin, D. C., Kalgutkar, A. S., and Lanzo, C. A.  1999.  Arachidonic acid oxygenation by COX-1 and COX-2: Mechanisms of catalysis and inhibition.  J. Biol. Chem. 274, 22903-22906.

 

9.  Marnett, L. J., Goodwin, D. C., Rowlinson, S. W., Kalgutkar, A. S., and Landino, L. M.  1999.  Structure, function, and inhibition of prostaglandin endoperoxide synthase. In Comprehensive Natural Products Chemistry, Vol. V pp. 225-261. C.D. Poulter, Ed. Elsevier Science, Amsterdam.

 

8.  Goodwin, D. C., Gunther, M. R., Hsi, L. C., Crews, B. C., Eling, T. E., Mason, R. P., and Marnett, L. J.  1998.  Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover: detection of the radical derivative of tyrosine 385. J. Biol. Chem. 273, 8903-8909.

 

7.  Goodwin, D. C., Grover, T. A., and Aust, S. D.  1997.  Roles of efficient substrates in peroxidase-catalyzed oxidations. Biochemistry 36, 139-147.

 

6.  Goodwin, D. C., Grover, T. A., and Aust, S. D.  1996.  Redox mediation in the peroxidase-catalyzed oxidation of aminopyrine: possible implications for drug-drug interactions. Chem. Res. Toxicol. 9, 476-483.

 

5.  Goodwin, D. C., Aust, S. D., and Grover, T. A.  1996.  Free radicals produced during oxidation of hydrazines by hypochlorous acid.  Chem. Res. Toxicol. 9, 1333-1339.

 

4.  Goodwin, D. C., Aust, S. D., and Grover, T. A.  1995.  Evidence for veratryl alcohol as a redox mediator in lignin peroxidase-catalyzed oxidation.  Biochemistry 34, 5060-5065.

 

3.  Goodwin, D. C., Yamazaki, I., Aust, S. D., and Grover, T. A.  1995.  Determination of transient-state rate constants for rapid peroxidase reactions. Analyt. Biochem. 231, 333-338.

 

2.  Goodwin, D.C., Barr, D. P., Aust, S. D., and Grover, T. A.  1994.  The role of oxalate in lignin peroxidase catalyzed reduction: Protection from compound III accumulation.  Arch. Biochem. Biophys. 315: 267-272.

 

1.  Goodwin, D.C., and Lee, S. B.  1993.  Rapid, microwave mini-prep of total genomic DNA from fungi, plants, protists and animals for PCR.  Biotechniques 15: 438- 444.

 

E. Patents and Inventions

 

Varnado, C., Olson, J. S.,  and Goodwin, D. C. Increasing recombinant Hemoglobin expression for blood substitute production in E. coli by Co-Expression with the heme receptor gene (chuA) from E. coli 0157:H7.  Invention Disclosure Filed with Rice University.

 

Goodwin, D. C., and Varnado, C. System for the Expression of Recombinant Hemoproteins in Escherichia coli.  U.S. Provisional Patent # 60/375,347.

 

F.  Presentations, Meetings, and Abstracts

 

Cook, C.O., Moore, R.L., and Goodwin, D. C. 2008.  Role of R117 and D597 interdomain residues in the reactivation of E. coli catalase-peroxidase.  American Chemical Society, 235th National Meeting, New Orleans, LA.

 

Cook, C.O., and Goodwin, D.C.  2008.  Role of the central hydrogen bonding network interdomain residues in the bifunctionality of catalase-peroxidases.  NOBCChE 35th Annual Conference, Philadelphia, PA.

 

Cook, C.O., Moore, R.L., Goodwin, D.C. 2007. Effect of Distant, Intradomain Residues on Restoring the Catalase-Peroxidase Bifunctional Active Site, Southeast Regional Meeting of the American Chemical Society, Greenville, SC.

 

Moore, R.L., Williams, R., and Goodwin, D.C. 2007.  Role of interdomain interaction of tyrosine 111 on catalase-peroxidase , Southeast Regional Meeting of the American Chemical Society, Greenville, SC.

 

Whitley, E.M., Goodwin, D.C., Cupp, M.S., Todd, L.W., Zhang, D., Mount, J.D., Powell, L.J., and Cupp, E.W.  2007.  Conformational and functional stability and immunogenicity of a vasoactive insect salivary protein, Experimental Biology Annual Meeting, Washington, DC.

 

Varnado, C.L., Olson, J.S., and Goodwin D.C. 2007.  Expression of recombinant hemoproteins in E. coli using a heme protein expression system, 51st Annual Meeting of the Biophysical Society, Baltimore, MD.

 

Goodwin, D.C., Cook, C.O., Moore, R.L. 2007.  Roles of distant but highly conserved interactions in maintaining active site function in catalase-peroxidases. Gordon Conference: Enzymes, Coenzymes, and Metabolic Pathways, Biddeford, ME.

 

Cook, C. O., Moore, R. L., Goodwin, D. C. 2007. Role of intrasubunit interactions between domains in catalase-peroxidase structure and activity. American Chemical Society, 233rd National Meeting, Boston, MA.

 

Department of Chemistry, Jacksonville State University, February 20, 2007.  ³Structures and Mechanisms of Hemoproteins:  Implications for Enzyme Engineering, Bacterial Virulence, and Antibiotic Resistance².

 

Goodwin, D. C., Cook, C. O., Baker, R. D.  2006.  Modulation of catalase-peroxidase active site structure and catalysis by distant protein structures.  American Chemical Society, 231st National Meeting, Atlanta, Georgia.

 

Moore, R., Goodwin, D. C., Laband, K. A., and Powell, L.  2006. Role of interdomain salt bridge on catalase-peroxidase activity.  American Chemical Society, 231st National Meeting, Atlanta, Georgia.

 

Cook, C. O., Baker, R., and Goodwin, D. C.  2006.  Function of a gene-duplicated domain in catalase-peroxidase structure and activity.  American Chemical Society, 231st National Meeting, Atlanta, Georgia.

 

Department of Chemistry and Physics, Georgia College and State University, Milledgeville, GA, February 2006. Contribution of Protein Structural Features to the Unique Catalytic Properties of Catalase-Peroxidases:  Implications for Bacterial Virulence, Antibiotic Resistance, and Enzyme Engineering.

 

Department of Chemistry, University of West Florida, Pensacola, FL, October 21, 2005. Contribution of Protein Structural Features to the Unique Catalytic Properties of Catalase-Peroxidases:  Implications for Bacterial Virulence, Antibiotic Resistance, and Enzyme Engineering.

 

Li, Y., and Goodwin D. C.  2004.  Central participation of an interhelical insertion in catalase-peroxidase bifunctionality and resistance to peroxide-dependent inactivation. Southeast Regional Meeting of the American Chemical Society, Research Triangle Park, North Carolina.

 

Baker, R. D., Cook, C. O., and Goodwin D. C.  2004.  Essential contribution of the C-terminal domain to the structure of the catalase-peroxidase active site.  Southeast Regional Meeting of the American Chemical Society, Research Triangle Park, North Carolina.

 

Cook, C. O., Baker, R. D., and Goodwin, D. C.  2004.  Catalase-peroxidase active site restructuring by a distant and inactive domain.  Southeast Regional Meeting of the American Chemical Society, Research Triangle Park, North Carolina.

 

Laband, K. A., Baker, R. D., and Goodwin, D. C.  2004.  Contributions of an interdomain ion pair to the bifunctional properties of catalase-peroxidases.  Southeast Regional Meeting of the American Chemical Society, Research Triangle Park, North Carolina.

 

Varnado, C. L., and Goodwin D. C.  2004.  Heme- and peroxide-dependent formation of a novel crosslink in catalase-peroxidases.  Southeast Regional Meeting of the American Chemical Society, Research Triangle Park, North Carolina.

 

Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, November 1, 2004. Protein Structural Contributions to the Unique Catalytic Properties of Catalase-Peroxidases.

 

Department of Biochemistry, Wake Forest University, Winston-Salem, NC, August 3, 2004.  Protein Structural Contributions to the Unique Catalytic Properties of Catalase-Peroxidases.

 

Goodwin, D. C., Baker, R. D., Cook, C. O., and Laband, K. A. 2004.  Integral Involvement of an Inactive Domain in Catalase-Peroxidase Structure and Catalysis  Gordon Conference:  Enzymes, Coenzymes, and Metabolic Pathways, Meriden, NH, July 20.

 

Department of Chemistry, New York University, New York, NY, April 26, 2004. Protein Structural Contributions to the Unique Catalytic Properties of Catalase-Peroxidases.

 

Department of Biochemistry, Vanderbilt University, Nashville, TN, March 26, 2004. Structural Requirements for the Hemoprotein-Dependent Decomposition of Hydroperoxides:  Lessons from the Catalase-Peroxidases.

 

Department of Chemistry and Physics, LaGrange College, LaGrange, GA.  March 10, 2004.  Hemoprotein Structure and Mechanism: Implications for Enzyme Engineering, Bacterial Virulence, and Antibiotic Resistance.

 

Department of Chemistry, Butler University, Indianapolis, IN.  February 18, 2004.  Structural Requirements for the Hemoprotein-Dependent Decomposition of Hydroperoxides:  Lessons from the Catalase-Peroxidases.

 

Department of Chemistry, Case Western Reserve University, Cleveland, OH.  February 6, 2004.  Structural Requirements for the Hemoprotein-Dependent Decomposition of Hydroperoxides:  Lessons from the Catalase-Peroxidases.

 

Department of Chemistry and Physics, Georgia College and State University, Milledgeville, GA.  February 2, 2004. Hemoprotein Structure and Mechanism: Implications for Enzyme Engineering, Bacterial Virulence, and Antibiotic Resistance.

 

Goodwin, D. C., Baker, R. D., Varnado, C. L., and Li, Y.  2003.  Structural Requirements for the Hemoprotein-Dependent Decomposition of Hydroperoxides:  Lessons from the Catalase-Peroxidases.  Symposium Title: Chemistry and Biology of Oxidative Damage.  Southeast Regional Meeting of the American Chemical Society, Atlanta, GA.

 

Varnado, C. L., and Goodwin, D. C. 2003.  Characterization of a Novel Periplasmic Catalase-Peroxidase. Southeast Regional Meeting of the American Chemical Society, Atlanta, Georgia.

 

Baker, R., and Goodwin D. C. 2003. Insight into the Role of the C-Terminal Domain in Catalase-Peroxidase Function.  Southeast Regional Meeting of the American Chemical Society, Atlanta, Georgia.

 

Laband, K. A., and Goodwin D. C. 2003. Peroxidase-Catalyzed Oxidation of Plant-Derived o-Methoxyphenols:  Implications for the Metabolism of Health-Promoting Phenolic Compounds.  Southeast Regional Meeting of the American Chemical Society, Atlanta, Georgia.

 

Li, Y., and Goodwin D. C. 2003. Use of Deletion Mutagenesis to Determine the Novel Functions of Two Unique Interhelical Insertions in Catalase-Peroxidases.  Southeast Regional Meeting of the American Chemical Society, Atlanta, Georgia.

 

Goodwin, D. C., and Hertwig, K. M.  2003. Transient- and Steady-State Kinetics of Peroxidase-Catalyzed Capsaicinoid Oxidation.  Session Title: Phenolics in Foods and Natural Health Products.

 

Goodwin, D. C., Baker, R., and Li, Y.  2003. Protein Structural Contributions to the Unique Catalytic Properties of Catalase-Peroxidases.  American Chemical Society, 226th National Meeting, New York, NY.   Sept. 7, 2003; Bioinorganic Chemistry.

 

Baker, R., and Goodwin, D. C.  2003.  Essential Role of the C-terminal Domain in Catalase-Peroxidase Function.  American Chemical Society, 226th National Meeting, New York, NY.  Sept 9, 2003; Bioinorganic Chemistry.

 

Li, Y., and Goodwin, D. C. 2003. Roles of Two Interhelical Insertions in Catalase-Peroxidase Bifunctionality.  American Chemical Society, 226th National Meeting, New York, NY.  Sept 9, 2003; Bioinorganic Chemistry.

 

Varnado, C. L., Hertwig, K. M., Thomas, R., Roberts, J. K., and Goodwin, D. C. 2003. Spectral and Kinetic Properties of a Novel Periplasmic Catalase-Peroxidase.  American Chemical Society, 226th National Meeting, New York, NY.  Sept 10, 2003; Biological Chemistry

 

Goodwin, D. C., Baker, R., Varnado, C. L., and Li, Y.  2003. Protein Structural Contributions to the Unique Catalytic Properties of Catalase-Peroxidases.   Gordon Conference: Enzymes, Coenzymes, and Metabolic Pathways, Meriden, NH, July 14-17, 2003.

 

Goodwin D. C.  Hemoprotein Structure and Mechanism: Implications for Enzyme Engineering, Bacterial Virulence, and Antibiotic Resistance.  Department of Chemistry, State University of West Georgia, Carrollton, GA.  April 11, 2003.

 

Varnado, C. L., and Goodwin, D. C. 2002.  Design of a specialized expression system for recombinant hemoproteins. Southeast Regional Meeting of the American Chemical Society, Charleston, South Carolina, November 14, 2002.

 

Baker, R., Li, Y., and Goodwin D. 2002. Selective Elimination of Catalase Activity from Catalase-Peroxidase by Deletion Mutagenesis.  Southeast Regional Meeting of the American Chemical Society, Charleston, South Carolina, November 15, 2002.

 

Goodwin, D. C.  Hemoprotein Structure and Mechanism: Implications for Enzyme Engineering, Bacterial Virulence, and Antibiotic Resistance. Department of Chemistry, University of Mississippi, Oxford, MS.  October 18, 2002.

 

Goodwin, D. C., Li, Y., and Baker, R.  2002.  Selective Elimination of Catalase Activity from Catalase-Peroxidase by Deletion Mutagenesis.  Gordon Conference: Enzymes, Coenzymes, and Metabolic Pathways, Meriden, NH, July 23 and 24, 2002.

 

Hertwig, K. M., and Goodwin, D. C. 2002.  Characterization of a novel, extracellular catalase-peroxidase from E. coli O157:H7.  Council on Undergraduate Research, Posters on the Hill Forum, Washington, D.C.  April 18, 2002.

 

Hertwig, K. M. and Goodwin, D. C. 2001.  Cloning, overexpression, purification, and characterization of catalase/peroxidase from enterohemorrhagic E. coli O157:H7.  Amer. Chem. Soc. Southeast Regional Meeting, Savannah, GA.

 

Li, Y., Melius, P., and Goodwin, D. C. 2001. Activation of Bacterial Catalase-Peroxidases by Addition of Hemin.  Amer. Chem. Soc., Southeast Regional Meeting, Savannah, GA.

 

Hertwig, K. and Goodwin, D. C. 2001.  Cloning, overexpression, purification, and characterization of catalase/peroxidase from enterohemorrhagic E. coli O157:H7. Amer. Chem. Soc. 222nd National Meeting, Chicago, IL.

 

Goodwin, D. C.  Catalase/Peroxidase Structure, Function, and Kinetics:  Implications for Antibiotic Resistance and Bacterial Virulence.  Department of Chemistry, State University of West Georgia, Carollton, GA.  April 27, 2000.

 

Goodwin, D. C.  Mechanisms of Prostaglandin Biosynthesis:  Generation and Trapping of Tyrosyl Radicals. Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX.  April 26, 1999.

 

Goodwin, D. C.  Structure and Function of Catalase/Peroxidases:  Implications for Antibiotic Resistance and Virulence. Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX.  April 27, 1999.

 

Goodwin D. C.  Mechanisms of Prostaglandin Biosynthesis:  Generation and Trapping of Tyrosyl Radicals. Department of Chemistry, Auburn University, Auburn, AL.  January 29, 1999.

 

Goodwin, D. C.  Structure and Function of Catalase/Peroxidases:  Implications for Antibiotic Resistance and Virulence. Department of Chemistry, Auburn University, Auburn, AL.  April 26, 1999.

 

Goodwin, D.C., Rowlinson, S. W., and Marnett, L. J. 1998.  Heme oxidation states in prostaglandin endoperoxide H synthase catalytic mechanism. Amer. Chem. Soc. 216th National Meeting, Boston, MA.

 

Rowlinson, S.W., Crews, B. C., Goodwin D. C., and Marnett L. J. 1998. Structure/function analysis on the cyclooxygenase channel of mouse prostaglandin endoperoxide synthase-2. Amer. Chem. Soc. 216th National Meeting, Boston, MA.

 

Goodwin, D.C., Gunther, M. R., Hsi, L. C., Crews, B. C., Eling, T. E., Mason, R. P., and Marnett, L. J. 1997. Nitric oxide trapping of the Y385 radical during prostaglandin endoperoxide synthase turnover. Intl. Congress Biochemistry Mol. Biol./Amer. Soc. Biochemistry Mol. Biol. Joint Meeting, San Francisco, CA.

 

Goodwin, D. C.  Kinetics of redox mediation in peroxidase catalysis:  implications for metabolism of xenobiotics.  Department of Biochemistry, Center in Molecular Toxicology, School of Medicine, Vanderbilt University, Nashville, TN.  April 2, 1996.

 

Goodwin, D. C.  Kinetics of redox mediation in peroxidase catalysis:  implications for metabolism of xenobiotics.  Atherosclerosis, Nutrition, and Lipid Research Division, School of Medicine, Washington University, Saint Louis, MO, April 4, 1996. 

 

Goodwin, D. C.  Kinetics of redox mediation in peroxidase catalysis:  implications for metabolism of xenobiotics.  Department of Biochemistry, Michigan State University, East Lansing, MI, April 8, 1996.

 

Goodwin, D.C., Aust, S. D., and Grover, T. A.  1996.  Enhancement of peroxidase-catalyzed oxidation of hydrazine derivatives by chlorpromazine. Amer. Soc. Biochemistry Mol. Biol. National Meeting, New Orleans, LA.

 

Goodwin, D.C., Aust, S. D., and Grover, T. A.  1996.  Enhancement of peroxidase-catalyzed xenobiotic oxidation by phenothiazines. Soc. Toxicol. Annual Meeting, Anahiem, CA.

 

Goodwin, D.C., Aust, S. D., and Grover, T. A.  1995.  Phenothiazines as redox mediators in peroxidase-catalyzed xenobiotic oxidation. Soc. Toxicol. Mountain West Chapter Annual Meeting, Ft. Collins, CO.

 

Goodwin, D. C., Yamazaki, I., Aust, S. D., and Grover, T. A.  1995.  Determination of transient-state rate constants for peroxidase reactions. Amer. Soc. Biochemistry Mol. Biol./Amer. Chem. Soc. Div. Biol. Chem. National Meeting, San Francisco, CA.

 

Goodwin, D.C., Aust, S. D., and Grover, T. A.  1995.  Redox mediators in lignin peroxidase catalysis: A kinetic model. Amer. Soc. Biochemistry Mol. Biol./Amer. Chem. Soc. Div. Biol. Chem. National Meeting, San Francisco, CA.

 

Grover, T.A., Goodwin, D. C., Barr, D. P., and S.D. Aust.  1994.  Protection of lignin peroxidase activity: oxalate and cation radicals. Intl. Soc. Free Rad. Res. 7th Biennial Meeting, Sydney, Australia.

 

Goodwin, D.C., Aust, S. D., and Grover, T. A.  1994.  Veratryl alcohol (VA) mediated oxidation by lignin peroxidase. Intl. Soc. Free Rad. Res. 7th Biennial Meeting, Sydney, Australia.

 

Goodwin, D.C., Barr, D. P., Aust, S. D., and Grover, T. A.  1994.  Inactivation of lignin peroxidase of Phanerochaete chrysosporium by oxygen radicals. Amer. Soc. for Microbiol. 94th General Meeting, Las Vegas, NV.

 

Goodwin, D. C., Barr, D. P., Aust, S. D., and Grover, T. A.  1994.  The novel role of the fungal metabolite oxalate in the catalytic cycle of lignin peroxidase. Amer. Soc. Microbiol. Intermountain Branch Annual Meeting, Provo, UT.

 

Goodwin, D. C., Johnston, C. G., Aust, S. D., and Grover, T. A.  1993.  Microwave extraction of DNA from fungi in soil: a simple, rapid method for polymerase chain reaction. Amer. Soc. Microbiol., 93rd General Meeting, Atlanta, GA.

 

Johnston, C.G., Goodwin, D. C., and Aust, S. D. 1994. Use of ribosomal DNA for species delineation and detection of Phanerochaete spp. Amer. Soc. Microbiol. 94th General Meeting, Las Vegas, NV.

 

Goodwin, D.C., and Lee, S. B. 1992. Ribosomal DNA sequences of Leptomitus lacteus, Sapromyces elongatus, Aquilinderella fermentans, and Rhipidium sp. and  their evolutionary implications for the Oomycete order Leptomitales. Soc. Study Evol., University of California, Berkeley, CA.

 

G.  Research Support

 

National Science Foundation (MCB-0641614)

PI:  Goodwin, D. C.

Dates:  7/1/07 – 6/30/10

Indispensable Roles of an Inactive Domain in Catalase-Peroxidase Catalysis:  Applications for Enzyme Engineering

 

United States Department of Agriculture, Hatch Grant and Alabama Agricultural Experiment Station Special Grants Program.

PD: Elizabeth Whitley.  Co-PDs:  Dwight Wolfe, M Edmonson, Douglas C. Goodwin, , Development of Vaccines Targeting Horn Flies

 

USDA CSREES Special Research Grants

PI: Wolfe, D.  Co-PI¹s:  Goodwin, D. C., Whitley, E., and Abrams, M.

                              Dates: 8/1/06 – 7/31/07)

Evaluation of a Horn Fly Polyvalent Vaccine for Cattle

A protective vaccine for cattle against horn flies is being developed.  The antigens are derived from salivary proteins produced by this insect.  The Goodwin group is using CD and other techniques to evaluate structure and stability of the component proteins and gain insight into the function of these proteins in blood feeding.

Role: Co-PI

 

PRISM 2004

PI:  Goodwin, D. C.        Co-PI¹s:  Ellis, H. R., Duin, E. C., Striegler, S., and Singh, N.

Dates:  4/1/04 – 3/31/05

Circular Dichroism at Auburn University

Funds were requested for the purchase of a circular dichroism spectropolarimeter with Peltier temperature control system and autotitration system.

Role:  Principal Investigator

                             

                              PRF # 38802 – G4              PI:  Goodwin, D. C.        Dates: 1/1/2003 – 9/30/2005

Understanding the Bifunctional Active Site of Catalase-Peroxidases: Insights for Enzyme Engineering.

In these studies, deletion mutagenesis is used to identify the role of catalase-peroxidase-unique structural components in the bifunctional catalytic abilities of catalase-peroxidases. 

Role: Principal Investigator

 

Biogrant 2-1319200         PI:  Goodwin, D. C.        Dates 5/1/2001 – 4/30/2003

Alabama Biogrant through Auburn University

Mechanisms of Heme Acquisition by Enterohemorrhagic E. coli strain O157:H7.

In these studies, investigators seek to evaluate the influence of perturbations in heme structure on heme binding by a receptor isolated from enterohemorrhagic E. coli.

Role: Principal Investigator

 

H. Graduate Student Advisees

 

Current:

 

Carma O. Cook, Ph.D. Candidate, Chemistry, Project: Reactivation of the catalase-peroxidase N-terminal domain.

 

Shalley Kudalkar, Ph.D. Student, Chemistry, Project: Catalase-peroxidase N-terminal domain structural analysis.

 

Robert Moore, Ph.D. Candidate, Chemistry, Project: Interdomain interactions in catalase-peroxidases and their impact on active site function.

 

Yu Wang, Ph.D Student, Chemistry, Project: Function of the catalase-peroxidase C-terminal domain.

 

Previous:

 

Ruletha Baker, Ph.D. Graduate, Chemistry, Dissertation Title:  Roles of an inactive domain in in catalase-peroxidase catalysis: Modulation of active site architecture and function by gene duplication.  Defense date: August 14, 2006.

 

Cornelius Varnado, Ph.D. Graduatea, Chemistry, Dissertation Title:  Enhancing expression of recombinant hemoproteins: Progress toward understanding structure/function and therapeutic application.  Defense date: June 29, 2006.

 

Yongjiang Li, Ph.D., Chemistry, Dissertation Title:  Roles of two interhelical insertions in catalase-peroxidase catalysis: Tracing the impact of peripheral protein structures on heme enzyme function.  Defense date: July 27, 2005.  Current Position:  Postdoctoral Research Associate, National Institutes of Health.

 

Ronald Marcy, GND Graduate Student, Chemistry, Project:  Structure and function of catalase-peroxidases.  Progress:  Ron is an instructor at Alabama Southern Community College who worked in the laboratory to get exposure to the latest techniques in biochemistry and molecular biology in order to enhance teaching of his chemistry and microbiology courses.

 

I.  Graduate Student Ad Hoc Committee Member (Current in Italics)

 

Chengdong Huang, Ph.D. Student, Chemistry, Major Professor: Dr. Smita Mohanty

 

Russell Carpenter, Ph.D. Candidate, Chemistry, Major Professor: Dr. Holly Ellis

 

Erin Imsand, Ph.D. Student, Chemistry, Major Professor: Dr. Holly Ellis

 

Weiya Xu, Ph.D. Candidate, Chemistry, Major Professor: Dr. Evert Duin

 

Sidharth Venkatesh, Ph.D. Candidate, Chemical Engineering, Major Professor: Dr. Mark Byrne

 

Weikuan Li, Ph.D. Candidate, Chemistry, Major Professor: Dr. Stewart Schneller

 

Trafina Jadhav, Ph.D. Student, Biological Sciences, Major Professor: Dr. Marie Wooten

 

Matthew Goodwin, Ph.D. Student, Health and Human Performance, Major Professor: Dr. Bruce Gladden

 

James Harris, Ph.D. Candidate, Health and Human Performance, Major Professor: Dr. Bruce Gladden

 

Jody Burks, Ph.D. Student, Animal Science, Major Professor:  Dr. Jacek Wower

 

Mi Wang, Ph.D. Candidate, Chemistry, Major Professor:  Dr. Evert Duin

 

Xuanzhi Zhan, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Holly Ellis

 

Rajesh Gupta, Ph.D. Graduate, Chemical Engineering, Major Professor: Dr. Yoon Lee

 

John-Ryan McAnnally, M.S. Graduate, Biological Sciences, Major Professor: Dr. James Bradley

 

Kholis Abdurachim, Ph.D. Graduate, Chemistry Major Professor:  Dr. Holly Ellis

 

Dolapo  Adedji, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Evert Duin

 

Na Yang, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Evert Duin

 

Honglei Sun, M.S. Graduate, Chemistry, Major Professor:  Dr. Holly Ellis

 

Benlian Gao, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Holly Ellis

 

Ling Tang, Ph.D. Graduate, Animal Science, Major Professor:  Dr. Werner Bergen

 

Rong Wu, Ph.D. Graduate, Chemistry, Major Professor:  Dr. S. D. Worley

 

Wei Ye, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Stuart Schneller

 

Darcy Goodwin, Masters Graduate, Animal and Dairy Sciences, Major Professor:  Dr. Skip Bartol

 

Amanda Bean, Ph.D. Graduate, Chemistry, Major Professor:  Dr. Thomas Albrecht-Schmitt

 

Ben Stronach, Masters Graduate, Animal and Dairy Sciences, Major Professor:  Dr. Werner Bergen

 

Wendy White, Masters Graduate, Biological Sciences, Major Professor:  Dr. Marie Wooten

 

Chunru Lin, Ph.D. Student (resigned), Biology, Major Professor:  Dr. Marie Wooten

 

 

J.  Graduate Courses Taught

 

                              BCHE 7200 Advanced Biochemistry I

                              BCHE 7220 Principles of Cellular and Molecular Enzymology

                              CHEM 646/ANSC 619 Metabolism and Biochemical Genetics

                              CHEM 640 Biochemistry

                              CHEM 7950 Biochemistry Seminar

                              CHEM 7930 Directed Individual Study

                              CHEM 7750 Formal Presentations in Chemistry

 

K.  Undergraduate Research Advisees (Current in Italics)

 

Robert Andrew Campbell, Undergraduate (Junior, Biochemistry) Auburn University, May 2008 – present.  Auburn University Cellular and Molecular Biosciences Summer Research Fellow.

 

Michelle Muldowney, Undergraduate (Junior, Biochemistry) Auburn University, August 2007 - present

 

JaRyce Nabors, Undergraduate (Junior, Biochemistry) Auburn University, August 2007 – May 2008.

 

Joey Russel, Undergraduate (Biomedical Sciences) Auburn University, January 2008 – May 2008.

 

W. C. (Dub) Davison, High School Student (Senior, McAdory High School) Auburn University Cellular and Molecular Biosciences Teaching Enhancement Program, June 2006. 

 

Jessica Williams, Undergraduate (Sophomore, Chemistry), Auburn University, May 2007 – August 2007.

 

Michael Love, Undergraduate (Junior, Chemistry), Auburn University, January 2007 – December 2007 (excluding summer months).

 

JaRyce Nabors, Undergraduate (Junior, Biochemistry) Auburn University May 2006 – August 2006.  EPSCoR Undergraduate Summer Research Fellow.

 

Jennifer Smith, Undergraduate (Junior, Biochemistry), Auburn University January 2006 – May 2006. 

 

Allan Bagget, Undergraduatee (Senior, Biomedical Sciences), Auburn University January 2006 – May 2006.

 

Grant Nelson, Undergraduate (Senior, Microbiology), Auburn University; January 2007 – March 2007.

 

Rachel Williams, Undergraduate (Sophomore, Chemistry), Auburn University; January 2007 – December 2007.

 

Justin Alex Taylor, Undergraduate (Junior, Chemistry), Auburn University; August 2006 – December 2006.

 

Luke Powell, Undergraduate (Senior, Biomedical Sciences), Auburn University; August 2005 – August 2006.  COSAM Research Fellow

 

Kimberley Laband, Undergraduate (Junior, Molecular Biology), Auburn University; May 2002 – May 2005.

 

Daniel Carter, Undergraduate (Senior, Biomedical Sciences), Auburn University, August 2004 – December 2004.

 

Byron Smith, Undergraduate (Senior, Biomedical Sciences), Auburn University, August 2004 – December 2004.

 

Stephen Pehler, Undergraduate (Junior, Molecular Biology), Auburn University; April 2004 – October 2004.

 

Tyson Kilpatrick, Undergraduate (Senior, Biomedical Sciences), Auburn University; January 2004 – May 2004.

 

Greyson McGowin, Undergraduate (Senior, Biomedical Sciences), Auburn University; May 2003 – September 2003.  CMB Research Fellow

 

Derek Fortson, Undergraduate (Senior, Biochemistry), Auburn University; August 2002 – May 2003.

 

Sarah Peaslee Undergraduate (Senior, Biochemistry), Auburn University; June 2002 – May 2003.

 

Melanie Oliver, Undergraduate (Senior, Microbiology) Auburn University; May 2002 – December 2002. 

 

J. Kenneth Roberts, Undergraduate (Senior, Biomedical Sciences), Auburn University; June 1, 2002 – May 10, 2003. 

 

Robert Thomas, Undergraduate, Asbury College, Wilmore, KY.;  May 15, 2002 – August 15 2002.

 

Kristin Hertwig, Undergraduate (Graduated, Biochemistry), Auburn University; January 15, 2000 – December 2002. 

 

Erika Schansberg, Undergraduate (Graduated, Microbiology), Auburn University; August 2001 – May 2002. 

 

Thomas Cash, Undergraduate (Graduated, Chemistry), Auburn University; January 2002 – May 2002. 

 

Matthew McIntyre, Undergraduate (Graduated, Biomedical Sci.), Auburn University; May 2001 – August 2001. 

 

Nancy Ruth Wilkins, Undergraduate (Graduated, Biochemistry), Auburn University; May 2001 – August 2001. 

 

Emily Brantley, Undergraduate (Graduated, Molecular Biology), Auburn University; June 2000 – December 2001. 

 

Juan Carmona, Undergraduate (Graduated, Molecular Biology), Auburn University; August 2000 – May 2001.

 

Amy Wainwright, Undergraduate (Graduated, Biochemistry), Auburn University; January 4, 2000 – June 6, 2000.

 

Randy Booth, Undergraduate (Graduated, Biochemistry) Utah State University; May 20, 1996 – August 20, 1996.

 

Joseph Benson, Undergraduate (Graduated, Biochemistry) Utah State University; May 20, 1995 - May 20, 1996. 

 

Curtis Takemoto, Undergraduate (Graduated, Political Science) Utah State University; May 20, 1994 – May 20, 1996.

 

L.  Undergraduate Courses Taught (*Graduate Students Enrolled From Other Depts.)

 

                              *BCHE 5180/6180 General Biochemistry I

                              *BCHE 5190/6190 General Biochemistry II

                              BIOL 4980 Undergraduate Research

                              CHEM 4901 Special Problems in Chemistry 

                              CHEM 4950 Undergraduate Seminar

                              CHEM 1030 Fundamentals of Chemistry I

                              CHEM 1020 Survey of Chemistry II

                              SCMH 1010 Concepts of Science