L. Bruce Gladden Professor 2129 Memorial Coliseum (334) 844-1466

Ph.D.: Zoology/Exercise Physiology, University of Tennessee, Knoxville (1976)

B.S.: Zoology, Chemistry Minor, University of Tennessee, Knoxville (1972)

Graduate Classes:

• KINE 7680 - Advanced Physiology of Exercise I
• KINE 7700 - Advanced Physiology of Exercise II
• KINE 8780 - Biochemistry of Exercise

Research Program:

Oxygen uptake on-kinetics.

• Hernández, Andrés, Matthew L. Goodwin, Nicola Lai, Marco E. Cabrera, James R. McDonald, and L. Bruce Gladden. Contraction-by-contraction VO₂ and computer-controlled pump-perfusion as novel techniques to study skeletal muscle metabolism in situ. Journal of Applied Physiology, In Press, 2010.
• Lai, Nicola, L. Bruce Gladden, Pierre G. Carlier, and Marco E. Cabrera. Models of muscle contraction and energetics. Drug Discovery Today 5(4):273-288, 2008.
• Zoladz, Jerzy A., L. Bruce Gladden, Michael C. Hogan, Zenon Nieckarz, and Bruno Grassi. Progressive recruitment of muscle fibers is not necessary for the slow component of VO₂ kinetics. Journal of Applied Physiology, 105:575-580, 2008.
• Lai, Nicola, Gerald m. Saidel, Bruno Grassi, L. Bruce Gladden, and Marco E. Cabrera. Model of oxygen transport and metabolism predicts effect of hyperoxia on canine muscle oxygen uptake dynamics. Journal of Applied Physiology 103:1366-1378, 2007.
• Grassi, Bruno, Michael C. Hogan, Kevin M. Kelley, Richard A. Howlett, and L. Bruce Gladden. Effects of nitric oxide synthase inhibition by L-NAME on oxygen uptake kinetics in isolated canine muscle in situ. Journal of Physiology 568(Pt 3):1021-1033, 2005.
• Sahlin, Kent, JesBak Sørensen, L. Bruce Gladden, Harry B. Rossiter, and Preben K. Pedersen.  Prior heavy exercise eliminates VO₂ slow component and reduces efficiency during submaximal exercise in humans.  Journal of Physiology 564:765-773, 2005.
• Grassi, Bruno, Michael C. Hogan, Paul L. Greenhaff, Jason J. Hamann, Kevin M. Kelley, William G. Aschenbach, Dumitru Constantin-Teodosiu, and L. Bruce Gladden.  VO₂ on-kinetics in dog gastrocnemius in situ following activation of pyruvate dehydrogenase by dichloroacetate.  Journal of Physiology 538:195-207, 2002.
• Grassi, Bruno, Michael C. Hogan, Kevin M. Kelley, William G. Aschenbach, Jason J. Hamann, Ronald K. Evans, Robin E. Pattillo, and L. Bruce Gladden. Role of convective O₂ delivery in determining VO₂ on-kinetics in canine muscle contracting at peak VO₂. Journal of Applied Physiology 89: 1293-1301, 2000.
• Grassi, Bruno, L. Bruce Gladden, Creed M. Stary, Peter D. Wagner, and Michael C. Hogan. Peripheral O₂ diffusion does not affect VO₂ on-kinetics in isolated in situ canine muscle. Journal of Applied Physiology 85: 1404-1412, 1998.
• Grassi, Bruno, L. Bruce Gladden, Michele Samaja, Creed M. Stary, and Michael C. Hogan. Faster adjustment of O₂ delivery does not affect VO₂ on-kinetics in isolated in situ canine muscle. Journal of Applied Physiology 85: 1394-1403, 1998.

Exercise metabolism/biochemistry of exercise/fatigue/blood flow.

• Howlett, Richard A., Kevin M. Kelley, Bruno Grassi, L. Bruce Gladden, and Michael C. Hogan. Caffeine administration results in greater tension development in previously fatigued canine muscle in situ. Experimental Physiology 90(6):873-879, 2005.
• Hogan, Michael C., Bruno Grassi, Michele Samaja, Creed M. Stary, and L. Bruce Gladden.  Effect of contraction frequency on the contractile and noncontractile phases of muscle venous blood flow.  Journal of Applied Physiology 95:1139-1144, 2003.
• Dobson, John L. and L. Bruce Gladden.  Effect of rhythmic tetanic skeletal muscle contractions on peak muscle perfusion.  Journal of Applied Physiology 94:11-19, 2003.
• Samaja, Michele, Sonia Allibardi, Giuseppina Milano, Gabriella Neri, Bruno Grassi, L. Bruce Gladden, and Michael C. Hogan. Differential depression of myocardial function and metabolism by lactate and H⁺. American Journal of Physiology: Heart and Circulatory Physiology 276: H3-H8, 1999.
• Hogan, Michael. C., L. Bruce Gladden, Bruno Grassi, Creed M. Stary, and Michele Samaja. Bioenergetics of contracting skeletal muscle after partial reduction of blood flow. Journal of Applied Physiology 84: 1882-1888, 1998.
• Portington, Kevin J., David D. Pascoe, Michael J. Webster, Layne H. Anderson, Rodney R. Rutland, and L. Bruce Gladden. Effect of induced alkalosis on exhaustive leg press performance. Medicine and Science in Sports and Exercise 30: 523-528, 1998.
• Pascoe, David D. and L. Bruce Gladden. Glycogen resynthesis follwing short term high intensity exercise and resistance exercise. Sports Medicine 21:98-118, 1996.
• Hogan, Michael C., L. Bruce Gladden, Sadi Kurdak, and David C. Poole. L-(+)-lactate infusion into submaximally working dog gastrocnemius: increased muscle [lactate] reduces tension development independent of pH. Medicine and Science in Sports and Exercise 27: 371-377, 1995.

Lactate metabolism.

• Gladden, L. Bruce. 200th Anniversary of lactate research in muscle. Exercise and Sports Sciences Reviews 36:109-115, 2008.
• Gladden, L. Bruce. A “lactatic” perspective on metabolism. Medicine and Science in Sports and Exercise 40:477-485, 2008.
• Gladden, L. Bruce. Perspectives. Is there an intracellular lactate shuttle in skeletal muscle? Journal of Physiology 582:899, 2007.
• Goodwin, Matthew L., Andres Hernandez, James E. Harris, and L. Bruce Gladden. Blood lactate measurements and analysis during exercise: A guide for clinicians. Journal of Diabetes Science and Technology 1(4):558-569, 2007.
• Gladden, L. Bruce.  Lactate metabolism:  a new paradigm for the third millennium.  Journal of Physiology 558:5-30, 2004.
• Gladden, L. Bruce.  Lactate Metabolism during Exercise.  In Poortmans, J.R. (ed):  Principles of Exercise Biochemistry, 3rd, revised edition, Medicine and Sport Science, Basel, Karger, 2003, volume 46, pp. 152-196.
• Brooks, George A., and L. Bruce Gladden.  Chapter 8.  Metabolic Systems:  Anaerobic Metabolism (Glycolytic and Phosphagen).  In Tipton, C.M. (ed):  Exercise Physiology:  People and Ideas.  New York:  Oxford University Press, 2003, pp. 322-360.
• Kelley, Kevin M., Jason J. Hamann, Christine Navarre, and L. Bruce Gladden.  Lactate metabolism in resting and contracting canine skeletal muscle with elevated lactate concentration.  Journal of Applied Physiology 93:865-872, 2002.
• Hamann, Jason J., Kevin M. Kelley, and L. Bruce Gladden.  Effect of epinephrine on net lactate uptake by contracting skeletal muscle.  Journal of Applied Physiology 91:2635-2641, 2001.
• Gladden, L. Bruce. Lactic acid: New roles in a new millenium. Proceedings of the National Academy of Sciences 98: 395-397, 2001.
• Gladden, L. Bruce. Muscle as a consumer of lactate. Medicine and Science in Sports and Exercise 32: 764-771, 2000.
• L. Bruce Gladden. Lactate transport and metabolism during exercise. In: Handbook of Physiology. Exercise: Regulation and Integration of Multiple Systems, edited by Loring B. Rowell and John T. Shepherd. New York: Oxford University Press, 1996, pp. 614-648.
• Hogan, Michael. C., L. Bruce Gladden, Sadi Kurdak, and David C. Poole. L-(+)- lactate infusion into submaximally working dog gastrocnemius: increased muscle [lactate] reduces tension development independent of pH. Medicine and Science in Sports and Exercise 27:371-377, 1995.
• L. Bruce Gladden, Robert E. Crawford, and Michael J. Webster. Effect of lactate concentration and metabolic rate on net lactate uptake by canine skeletal muscle. American Journal of Physiology: Regulatory Integrative and Comparative Physiology 35:R1095-R1101, 1994.
• Poole, David C., L. Bruce Gladden, Sadi Kurdak, and Michael C. Hogan. L-(+)- Lactate infusion into working dog gastrocnemius. No evidence that lactate per se mediates VO₂ component. Journal of Applied Physiology 76:787-792, 1994.
• L. Bruce Gladden, Robert E. Crawford, and Michael J. Webster. Effect of blood flow on net lactate uptake during steady-state contractions in canine skeletal muscle. Journal of Applied Physiology 72(5):1826-1830, 1992.
• L. Bruce Gladden. Net lactate uptake during progressive steady-level contraction in caninne skeletal muscle. Journal of Applied Physiology 71(2):514-520, 1991.

Lactate transport across muscle membrane.

• Evans, Ronald K., Dean D. Schwartz, and L. Bruce Gladden.  Effect of myocardial volume overload and heart failure on lactate transport into isolated cardiac myocytes.  Journal of Applied Physiology 94:1169-1176, 2003.
• Aschenbach, William G., Gregory L. Brower, Robert J. Talmadge, John L. Dobson, and L. Bruce Gladden.  Effect of a myocardial overload on lactate transport in skeletal muscle sarcolemmal vesicles. American Journal of Physiology: Regulatory, Integrative, and Comparative Physiology 281: R176-R186, 2001.
• L. Bruce Gladden, Robert E. Crawford, Michael J. Webster, and Peter W. Watt. Rapid tracer lactate influx into canine skeletal muscle. Journal of Applied Physiology 78:205-211, 1995.
• Watt, Peter W., L. Bruce Gladden, Harinder S. Hundal, and Robert E. Crawford. Effects of flow and contraction on lactate transport in the perfused rat hindlimb. American Journal of Physiology: Endocrinology and Metabolism 267:E7-E13, 1994.
  

Lactate transport by red blood cells.

• Pattillo, Robin E. and L. Bruce Gladden.  Red blood cell lactate transport in sickle disease and sickle cell trait.  Journal of Applied Physiology 99:822-827, 2005.
• Smith, Edith W., Michele S. Skelton, DuAnn E. Kremer, David D. Pascoe, and L. Bruce Gladden.  Lactate distribution in the blood during steady-state exercise.  Medicine and Science in Sports and Exercise 30: 1424-1429, 1998.
• Skelton, Michele S., DuAnn E. Kremer, Edith W. Smith, and L. Bruce Gladden. Lactate influx into red blood cells from trained and untrained human subjects. Medicine and Science in Sports and Exercise 30:536-542, 1998.
• Smith, Edith W., Michele S. Skelton, DuAnn E. Kremer, David D. Pascoe, and L. Bruce Gladden. Lactate distribution in the blood during progressive exercise. Medicine and Science in Sports and Exercise 29:654-660, 1997.
• Skelton, Michele S., DuAnn E. Kremer, Edith W. Smith, and L. Bruce Gladden. Lactate influx into red blood cells of 'athletic' and 'non-athletic' species. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 268:R1121-R1128, 1995.

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For additional information gladdlb@.auburn.edu