The Applied Biosystems 7500 Real Time PCR Systems uses fluorescent-based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and association-curve analysis. ABI Real Time PCR 7500 Chemistry: Applied Biosystems has developed two types of chemistries that can be used to detect PCR products using 7500 Real Time PCR Systems: 1) TaqMan Probe-based fluorogenic 5' nuclease chemistry. 2) SYBR Green I dye chemistry. Applications: ABI7500 Real Time PCR Systems can be used for: 1) Absolute Quantification: to determine the absolute quality of a single nucleic acid target sequence within an unknown sample. 2) Relative Quantification: to determine the change in expression of a nucleic acid sequence (target) in a test sample relative to the same sequence in a calibrator sample. 3) Plus/Minus Assay: to determine if a specific target sequence is present (plus) or not present (minus) in a sample. 4) Allelic Discrimination: to detect variants of a single nucleic acid sequence, SNP. ABI 7500 Real Time PCR Systems is located at 33 Life Science Building. Required User Supplied Materials: For using ABI 7500 Real Time PCR Systems, users need to provide their own 96 well plates (Applied Biosystems PN 4306757), Optical Adhesive Cover (Applied Biosystems PN 4311971), TaqMan RT-PCR reagents, TaqMan Universal PCR Master Mix (Applied Biosystems PN 4304437), TagMan Probe based probe or primers or SYBR Green based reagents, also tubes and tips for PCR reaction (for details please consult your specific application protocol or visit Applied Biosystems homepage). Price for using ABI 7500 Real Time PCR Systems: $6.00/hour Please fill out the order form if you are planning to use ABI 7500 Real Time PCR System. |
Auburn University | Auburn, Alabama 36849 | Phone: (334) 844-4000 | E-mail: © Copyright Regulations |
