Sending your samples to
run electrophoresis on the ABI Prism 3100 Genetic Analyzer
I. If you performed SNaPshot
reactions in your own lab, just send the final products to GSL.
II. For sending your samples
to conduct SNaPshot reaction in GSL:
1) Pooling PCR amplified
SNaPshot templates: if you have multiple PCR amplified samples to run
in a single well, mix equal volums (e.g 2 ul each) of these products
in a tube.
2) Pooling SNaPshot
primers: all the primers to be used in a single SNaPshot reaction should
be premixed to give a final concentration of 0.2 uM for each primer.