Sequencing Preparation Chart

PCR
100-200 bp
 200-500 bp
500-1000 bp
1000-2000 bp
>2000 bp
 
1-3 ng/ul
3-10 ng/ul
5-20 ng/ul
10-40 ng/ul
40-100 ng/ul

DNA concentration required for PCR products

Customer Provided Primers: Primer concentration should be 3 uM or 20 ng/ul. Please provide 8 ul of primer per reaction

AU GSL Primers: AU GSL provides following primers for sequencing
DNA Template
3-5 Kb
5-8 Kb
8-10 Kb
>10 Kb
Double Stranded
100-200 ng/ul
250-500 ng/ul
500-800 ng/ul
1 ug/ul
Single Stranded
 25-35 ng/ul
50-70 ng/ul
100-140 ng/ul
200 ng/ul

DNA concentration required for plasmid
Primer
Sequence
SP6 promoter primer
5'-GAT TTA GGT GAC ACT ATA G-3'
T3 promoter primer
5'-ATT AAC CCT CAC TAA AGG GA-3'
T7 promoter primer
5'-TAA TAC GAC TCA CTA TAG GG-3'
M13 Reverse primer
5'-TCA CAC AGG AAA CAG CTA TGA C-3'
M13 Forward primer
5'-TGT AAA ACG ACG GCC AGT-3'
 

Back to Sequencing Instructions Page

Back to GSL Home

Back to Research Instrumentation Home