Instructions

The Genomics & Sequencing Lab (GSL) uses the ABI 3100 Genetic Analyzer for the analysis of fluorescently labeled DNA fragments.The use of four flourescent dyes allows multi-plexing of three microsatellites (for example) plus a size standard in every lane, eliminating lane to lane variability and increasing throughput.The GeneScan software collects and sizes or quantitates the DNA fragments. The Genotyper software is available for additioinal genetic analysis of the data such as forensic identification, inheritence checking, linkage mapping, etc. The data output has peaks similar to sequencing and/or as tabular data for importing into a spreadsheet.