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GSL - HIA
Human
Identification Applications
Applied Biosystems has combined the advantages of PCR-based
testing and fluorescence detection into a highly discriminating kit designed for
human identification applications. The AmpFLSTR® Profiler Plus™ PCR
Amplification Kit amplifies nine tetranucleotide short tandem repeat (STR) loci
and the Amelogenin locus in a single reaction tube. The STR loci amplified are
D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and vWA. The
Amelogenin locus is used for gender identification because products of different
lengths are generated from the X and Y chromosomes
 The
AmpFLSTR® Profiler Plus™ reagents and protocols have been developed to produce
the quality results necessary for human identification applications. To increase
the success of obtaining results in the presence of enzyme inhibitors, bovine
serum albumin (BSA) has been included in the AmpFLSTR® PCR Reaction Mix.
Thermal cycler times and temperatures have been developed
to produce specific amplification while providing the necessary degree of
sensitivity. The recommended range of input DNA is 1.0 to 2.5 ng. Furthermore,
instrument detection protocols have been provided to yield reproducible results
with excellent resolution and precision in sizing alleles.
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