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GSL - AFLP
Amplified
Fragment Length Polymorphism
AFLP® is a DNA fingerprinting technique
which detects DNA restriction fragments by means of PCR amplification.
The AFLP® technology usually comprises of
the following steps:
The restriction of the DNA with two
restriction enzymes, preferably a hexa-cutter and a tetra-cutter;
The ligation of double-stranded (ds) adapters to the ends of the restriction
fragments;
The amplification of a subset of the restriction fragments using two primers
complementary to the adapter and restriction site sequences, and extended at
their 3' ends by "selective" nucleotides (see Figures 1 and 2);
Gel electrophoresis of the amplified restriction fragments on denaturing
polyacrylamide gels ("sequence gels");
The visualization of the DNA fingerprints by means of autoradiography, phospho-imaging,
or other methods. For more information about AFLP please visit:
http://www.keygene.com/keygene/techs-apps/index.php. | 
