Specifying Thermal Cycling Conditions and Starting the Run:

There is a default thermal cycling condition for the PCR step of the procedure:

1. Select the Instrument tab

2. Delete the default first stage by Shift+clicking near the bottom of the stage box to select it, then clicking delete,

change the temperature for the second step to 92

Sample volume is 25ul

9600 Emulation is selected. \

3. Select File>Save As, enter a name for the AQ Plate document, then click Save.

4. Load the plate into the instrument.

5. Click Start.

After finish the run perform the Post-Read Run:

Open the pre-read plate document

1. Select the Instrument tab.

2. Accept the defaults for: 50 ul sample, and select 9600 Emulation

3. Select File>Save As, type: AD Post-Read, then click Save

4. Click Post-Read

5. After the run is finished, click the green analysis button to start analysis and then Select the Results Tab and then select Allelic Discrimination Tab.