Office: 326 Life Sciences Building Laboratory: 334 Life Sciences Building Phone: (334) 844-1667/ 1645 (FAX) E-mail: singhna@auburn.edu
Ph.D. Molecular Biology
(1979) - Tata Institute of Fundamental Research, Mumbai, India M.S. Botany (1967) - Patna University, India B.S. Honors (1964) - Patna University, India
Professor (1995 -
present) – Auburn University, Alabama Associate Professor (1989
- 1995) – Auburn University, Alabama Associate Professor (1988
- 1989) – Virginia Commonwealth University, Virginia Research Associate (1982 - 1987) – Purdue University, Indiana Lecturer and Reader (1968
- 1982) – Ranchi University, India
Courses taught at Auburn University
Principles of Biology (BIOL 1020)
Honors Biology (BIOL 1027)
Plant Biology (BIOL 3200)
Plant Gene Expression (BIOL 5320/6320)
Introductory Molecular Genetics (BIOL 5220/6220)
Recombinant DNA and Gene Expression (BIOL 5521/6521)
Undergraduate seminars (BIOL 4950): Protein sorting,
Transgenics, Central dogma exceptions, Small regulatory RNA
Special topics (BIOL 7970): Stress proteins,
Molecular biology of plant development
Undergraduate research (BIOL 4980)
Special problems (BIOL 7980)
Major
professor of graduate students and their current affiliations
Yuying Sang, Ph.D. 2008: Assistant Professor, Academy of Sciences, Shanghai Institute of Plant Physiology and Environment, China
Nisreen Al-Quraan, Ph.D. 2008: Assistant Professor, Department of Biotechnology & Genetic Engineering, Jordan University of Science & Technology, Jordan
Kun Yuan, Ph.D. 2008: Assistant Professor, South China University of Science & Technology, Guandong, China (Co-advisor)
Janani Shutthanandan, M.S.2002: Research Associate, Pacific Northwest National Lab., Richland, WA
Quing Luo, M.S. 2000: Present position not known
Shu-Yi Wang, Ph.D. 2000: Present position not known
Sagadevan Mundree, Ph.D. 1996: Professor & Deputy Director, Center for Tropical Crops, Queensland University of Technology, Australia
Roland W. Herzog, Ph.D. 1996: Professor, Division of Cellular & Molecular Therapy, Department of Pediatrics, University of Florida, FL
Rajendra J. Redkar, Ph.D. 1995: Senior Scientist, Mol. Biol., Schott Glass Inc., Dureya, PA
Ching-Chun Chang, M.S. 1994: Associate Professor, Plant Sciences, National Cheng Kung University, Taiwan
Current research
interest
● Responses
to environmental stress:changes ingene expression profile in response to heat and draught
● Edible
vaccines:development
of plant derived edible vaccinesagainst viral diseases
Publications (1986-2011)
Pant, S.R., N.K. Singh, D. Weaver, C-C. Weng, M.
Zhong, and R.D. Locy. 2011. Changes in gene expression associated with heat stress in cotton as determined by deep
sequencing of cDNA libraries. Proc. Beltwide Cotton Conf. (In Press).
Hu, H, N.K.
Singh,
D. Weaver, Pant, S.R., M. Egnin, M. Zhong, C-C. Weng, and R.D. Locy. 2011.
Characterization of cotton leaf miRNA
and changes in miRNA expression associated with heat stress in cotton as
determined by deep sequencing. Proc. Beltwide.
Cooton. Conf. (In press).
Al-Quaraan, N.A., R.D. Locy, and N.K.
Singh.
2011. Implication of paraquat and hydrogen peroxide-induced oxidative stress treatments on the GABA shunt pathway in Arabidopsis thaliana
calmodulin mutants. Plant Biotech Rep. 5: 225-234.
Wu, H-Z, K. S.
Gunn, S.R. Singh, N.K. Singh,
S-C Ou, and J.J. Giambrone. 2011. DNA vaccine against H1N1 avian influenza virus-induced humoral and cell-mediated
immunity in SPF chickens. Jour Biotech Res. 3:27-36.
Wu, H-Z, K. S. Gunn, N.K.
Singh, S-C Ou, and J.J. Giambrone. 2011. Yeast-derived avian influenza virus hemagglutin protein induced immune response in SPF chicken. J.
Animal Vet Advances. 10(8): 999-1004.
Tzou, Y-M., T-S. Huang, B.A. Chin and N.K.
Singh.
2011. Expression of tobacco Osmotin in Escherichia coli for biocidal
activity. Biotechnology Letters. 33:539-543.
Sang, Y., R.D. Locy, L. Goertzen, Y-M, Tzou, K.Y.
Kang and N.K. Singh. 2011. Identification of a second pridoxamine
phosphate oxidase in Arabidopsis thaliana. Acta Physiol. Plant. 33:559-567.
Sang, Y., L. Goertzen, A. Rashotte, Y. Si, R.D.
Locy, and N.K. Singh. 2010. Expression, in vivo localization
and phylogentic analysis of
Arabidopsis Pyridoxine 5’-phosphate Oxidase. Plant Physiol. Biochem. 49:88-96.
Al-Quaraan, N.A., R.D. Locy,
and N.K. Singh. 2010. Expression of Calmodulin genes in wild type and Calmodulinmutants
of Arabidopsis thaliana under heat stress. Plant Phyiol. Biochem.
48:697-703.
Ying, S., F. Dane, A. Rashotte, K. Kang, and N.K.
Singh.
2010. Cloning and expression analysis of Ccrboh gene encoding respiratory burst oxidase in Citrullus
colocanthus and grafting onto Citrullus lanatus (watermelon). J.
Experimental Bot. 61:1635-1642.
Barbosa, J.M., N.K. Singh, J.H. Cherry and R.D. Locy.
2010. Nitrate uptake
and utilization is modulated by exogenous γ-aminobutyric
acid in Arabidopsis thaliana seedlings. Plant Physiol. Biochem.
48:443-450.
Wu, H-Z., K. Wiiliams, S.R.
Singh, K.S. Gunn, N.K. Singh, D. Teressa, and J.J. Giambrone. 2009. Integrity of
recombinant hemagglutinin protein of
Avian Influenza Virus H10N7. Biotechnology letters. 31: 1511-1517.
Wu, H-Z., K.S. Gunn, N.K.
Singh and
J.J. Giambrone. 2009. Development of plant based vaccine against reovirus.
Avian Diseases. 53: 376-381.
Parkhi,V., V.Kumar, G.Sunilkumar, L.M.Campbell, N.K.Singh
and K.S.Rathore. 2009.Expression of apoplastically-secreted tobacco osmotin in cotton confers drought
tolerance. Mol Breeding. 23:625-639.
Giambrone, J.J., T.
Dormitorio, H-Z. Wu, N.K. Singh. 2008. Development of a recombinant avian influenza
vaccine in yeast. Feedinfo. January,
2008.
Sang, Y., J. M. Barbosa,
H-Z, Wu, R.D. Locy and N.K. Singh. 2007. Identification of pyridoxine (pyridoxamine)
5’ phosphate oxidase from Arabidopsis
thaliana. FEBS Letters. 581: 344-349
Wu, H-Z., K.S. Gunn, N.K.
Singh,
R.D. Locy, and J.J. Giambrone. 2005. Yeast derived sigma C protein induced
immunity against avian reovirus.
Avian Disease. 49:281-285.
Zhang, C., L. Ping, F. Dane, R. Ebel, N.K. Singh,
R.D. Locy, and W. Dozier. 2005. Cold acclimation induced genes in trifoliate orange, Poncirus trifoliate. Plant
Cell Reporter 23:764-769.
Zhang, C., L. Ping, F. Dane, R. Ebel, N.K. Singh,
R.D. Locy, and W. Dozier. 2005. Down regulated gene expression of cold acclimated Poncirus trifoliate.
Can. J. Plant. Sci. 85: 417-424.
Wu, H-Z., N.K. Singh, R.D. Locy, K.S. Gunn, and
J.J. Giambrone. 2004. Immunization of chicken with VP2 protein of infectious bursal disease virus expressed in Arabidopsis
thaliana. Avian Disease 48:663-668.
Wu, H-Z., N.K.
Singh,
R.D. Locy, K.S. Gunn, and J.J. Giambrone. 2004. Comparison of avian reovirus S1
genes. Jour Anim. Vet. Adv.3:
326-329.
Wu, H-Z, N.K.
Singh, R.D. Locy, K.S. Gunn, and J.J. Giambrone. 2004. Expression of
immunogenic VP2 of IBDV in transgenic Arabidopsis thaliana. Biotechnology
letters 26: 787-792.
Lugo, S.K., M. Kunnimalayaan, N.K. Singh, and B.L. Nielsen. 2004. Required sequence
elements for chloroplast DNA replication activity
in vitro and in electroporated
chloroplast. Plant Sci. 166: 151-161.
Giambrone, J.J., N.K.
Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2003. Development of edible
transgenic plant vaccine for immunizing
poultry. Current Concept in Production. Pp. 1-3.
Narsimhan, M.L., H. Lee, B.
Damsz, N.K. Singh, J.I. Ibeas, T.K. Mastmuto, C.P. Woloshuk, and
R.A. Bressan.2003. Overexpression of
a cell wall glycoprotein in Fusarium
oxysporum increases virulence and resistance to a plant PR-5 protein. Plant Jour.
36: 390-400.
Giambrone, J.J., N.K.
Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2002. Edible inoculations:
developing edible transgenic plant vaccine
to immunize chicken against IBDV. AAES Highlights. 9: 1-3.
Giambrone, J.J., N.K.
Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2001. Development of edible
transgenic plant vaccine for immunizing
chicken against IBDV. Feedinfo.com. 3: 1-3.
Giambrone, J.J., N.K.
Singh, R.D. Locy, K. Scissum-Gunn, and H. Wu. 2001. Transgenic plant
vaccine to immunize chicken against
infectious Bursal disease virus. Highlights in Agriculture. 48 (4).
Wang, S-Y, S-J. Wu, G.
Thottappilly, R.D. Locy, and N.K. Singh. 2001. Molecular cloning
and structural analysis of the gene encoding
Bacillus cereus exochitinase Chi36. JBB 92:59-66.
Wang, S-Y, A-L. Moyne, G.
Thottappilly, S-J. Wu, R.D. Locy, and N.K. Singh. 2001.
Purification and characterization of a Bacillus cereus exochitinase. Enzyme
Microbial Technol. 28:492-498.
Wu, S-J., R.D. Locy, J.J. Shaw, J.H. Cherry and N.K. Singh. 2000. Mutation in ArabidopsisHIT1 locus causing heat and osmotic hypersensitivity.
J. Plant Physiol. 157:543-548.
Singh, N.K., S.G. Mundree, and R.D. Locy. 2000. Novel
determinants of salinity tolerance. In: Plant Tolerance to Abiotic Stresses in Agriculture: Role of Genetic Engineering
(eds. J.H. Cherry) Kluver Publication. pp 131-138.
Barbosa, J.M., R.D. Locy,
T.W. Barger, N.K. Singh and J.H. Cherry. 2000. GABA increases the
rate of nitrate uptake and utilization
in Arabidopsis roots. Ibid. pp. 53-63.
Locy, R.D., S-J. Wu, J.
Bistnette, T.W. Barger, D. McNabb, M. Zik, H. Fromm, M.R. Knight, N.K.
Singh and J.H. Cherry. 2000. The
regulation of GABA accumulation by heat stress-induced increases in cytoplasmic
calcium. Ibid. p. 39-52.
Thottappilly, G., H.D. Mingnouna, A. Onasanya, M.
Abang, O. Oyelakin, and N.K. Singh.
1999. Identification and differentiation of
isolates of Colletotrichum gleosporoides
from Yam by random amplified polymorphic DNA markers. African Crop Science Journal. 7: 195-205.
Singh,
N.K. 1999. Genetic transformation of edible fungi. Proc.
International Mushroom Conference. 49-53.
Redkar, R.J., R.W. Herzog and N.K. Singh. 1998. Transcriptional activation of the Aspergillus nidulans gpd promoter
by osmotic signals. Applied Env. Microbiol. 64:2229-2234.
Herzog. R.W., N.K.
Singh, D.W. Urry and H. Daniell. 1997.
Expression of synthetic protein based polymer (Elastomer) gene in Aspergillus
nidulans. Applied Microbiol. and Biotech. 47:368-372.
Johnson, B.S., N.K.
Singh, J.H. Cherry, and R.D. Locy. 1997. Purification and characterization of cowpea glutamate decarboxcylase. Phytochem. 46:39-44.
Chang, C.C., R.D. Locy, R. Smeda, S.V. Sahi and N.K. Singh. 1997.
Photoautotrophic tobacco cells adapted to grow in high salinity. Plant Cell Report. 16:495-502.
Chang, P.F., B. Damsz, A.K. Kononowicz, A. Chen, Yi
Xu, N.K. Singh, M.L.
Narshimhan, P.M. Hasegawa, and R.A.
Bressan. 1996. Alteration in cell membrane associated protein after
adaptation to osmotic stress. Physiol. Plant. 98:505-516.
Abed, L.R., D. Liu, M.P. Durzo, M.L. Narsimhan, N.K. Singh, P.M. Hasegawa and.
R.A. Bressan. 1996. The antifungal activity of osmotin
involves the fungal plasma membrane and cell wall. Plant Science. 118:11-23.
Chen, L., C.M. Peterson, and N.K. Singh. 1996. Isolation of cDNA clones from petiole
abscission zones of cotton. Proc. Belt. Cotton
Con. 2:1243-1246.
Redkar, R.J., P.A. Lemke and N.K. Singh. 1996.
Altered gene expression in Aspergillus nidulans in response to salt stress. Mycologia 88:256-263.
Herzog, R.W., N.K.
Singh, H. Daniell and P.A. Lemke.
1996. A comparative study on transformation of Aspergillusnidulans by microprojectile bombardment of conidia and protoplast treated
with polyethylene glycol. Applied
Microbiol. Biotech 45:333-339.
Locy, R.D., C.C. Chang, B.L. Nielsen and N.K. Singh. 1996. Photosynthesis
in salt-adapted heterotrophic tobacco cells and regenerated plants. Plant Physiol. 110:321-328.
Redkar, R.J., P.A. Lemke and N.K. Singh. 1996. Isolation of differentially expressed cDNA
clones from salt- adapted Aspergillus nidulans. Current Genetics.
29:130-135.
Redkar, R.J., R.D. Locy and N.K. Singh. 1995. Biosynthetic pathways of glycerol
accumulation under salt stress in Aspergillus nidulans. Experimental Mycology.
19:241-246.
Herzog, R.W., N.K.
Singh, C. Schmidt and P.A. Lemke. 1995. Presence of a P1 bacteriophage
sequence in transforming plasmids of Pleurotus ostreatus. Current Genetics.
27:460-465.
Lemke, P.A., N.K.
Singh and U-A Temann. 1994. Genetic transformation of Ectomycorrhizal
Fungi. In: Mycorrhiza: Structure, Function, Molecular Biology and
Biotechnology (eds.A. Varma and B. Hock). Springer-Verlag. pp. 137-156.
Kononowicz, A.K., K.G. Raghothama, A.M. Casas, D.E.
Nelson, D. Lieu, M.L. Narsimhan, P.C. LaRosa, N.K. Singh, R.A. Bressan and
P.M. Hasegawa. 1994. Structure,
regulation and function of osmotin gene. In: Biochemical and Cellular
Mechanisms of Stress Tolerance in
Plants (ed. J.H. Cherry) Springer-Verlag 381-414.
Cherry, J.H., R.M. Mayer, K. Heuss-LaRosa, P.M. Reddy
and N.K. Singh. 1994. Altered gene expression in
thermoadapted cultured cells of cowpea. Ibid. 229-242.
Daniell, H., C. Guda, N.K. Singh, J.D. Weete, J.H. Cherry. 1994. Photosynthesis, epiculticular wax and lipid
changes in cowpea cultivars grown
under hyperthermic conditions. Ibid. 213-227.
Smeda, R., P.M. Hasegawa, P.B. Goldsborough, N.K.Singh and S.C. Weller. 1993.
A serine to thrionine substitution in triazine herbicide-binding protein in potato cells results in atrazine
resistance without impairing productivity. Plant Physiol. 103:911-917.
Peng, M., P.A. Lemke and N.K. Singh 1993. A
nucleotide sequence involved in replicative transformation of a filamentous fungus.
Curr. Genet. 24:114-121.
Singh,
N.K. and J.J. Shaw. 1992.
Physical methods of gene transfer in plants. In Biotechnology:
Enhancing Research on Tropical Crops
in Africa. (Eds. G. Thottapilly, L. Monti, M. Raj and A. Moore). pp. 203-210.
LaRosa, P.C., Z. Chen, D.E. Nelson, N.K. Singh, P.M. Hasegawa and
R.A. Bressan. 1992. Osmotin gene
regulation is post-transcriptionally
regulated. Plant Physiol. 100:409-415.
Peng, M., N.K.
Singh and P.A. Lemke. 1992. Recovery of recombinant plasmids from Pleurotus ostreatus transformants. Curr. Genet.
22:53-59.
Lemke, P.A., N.K.
Singh, M. Peng. 1992. Genetic Engineering of an Edible Mushroom Species. AAES Highlights. 39:16
Nelson, D.E., K.G. Raghothama, N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1992.
Analysis of structure and transcriptional activation of the osmotin gene. Plant
Molec. Biol. 19:577-588.
Kononowicz, A.K., D.E. Nelson, N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1992. Regulation of the Osmotin gene promoter. Plant Cell 4:513-524.
Casas, A.M., D.E. Nelson, K.G. Raghothama, M.P. Durzo,
N.K. Singh, R.A. Bressan and
P.M. Hasegawa. 1992. Expression of OSM like
genes in the halophyte Atriplex
nummularia L. Plant Physiol. 99:329-337.
Charles, D.J., N.K.
Singh and J.E. Simon. 1992. The essential oils of Alpinia galangawild. Journ. Essential Oil Res. 4:81-83.
Narsimhan, M.C., M.L. Binzel, E. Perez-Prat, Z. Chen,
D.E. Nelson, N.K. Singh, R.A.
Bressan and P.M. Hasegawa. 1991. NaCl regulation of the gene encoding the 70 kDa subunit of tonoplast
ATPase in tobacco cells. Plant Physiol. 97:562-568.
Dylweski, D.P., N.K.
Singh and J.H. Cherry. 1991. Effects
of heat shock and thermo-adaptation the ultrastructure of Cowpea (Vigna
unguiculata) cells. Protoplasma. 163:125-135.
Pratt, R.C., N.K.
Singh, R.E. Shade and R.A. Bressan.
1990. Purification and
characteri-zation of isolectins from tepary bean (P. acutifolius) which
delay Bruchid Beetle development. Plant Physiol.
93:1453-1459.
Bressan, R.A., D.E. Nelson, N.M. Iraki, P.C. LaRosa, N.K. Singh, P.M. Hasegawa and
N.C. Carpita. 1990. Reduced cell
expansion and changes in cell walls
of plant cells adaptedto NaCl. In
Environmental Injury to Plants. F.
Katterman (ed.) Academic Press. pp, 137-171.
Carpita, N.C., N.K.
Singh, R.A. Bressan, P.M. Hasegawa, S.R. Schnapp. 1990. Cellular
mechanisms of salt and water stress tolerance
in plants. Acta Horticulturae.
280:341-352.
LaRosa, P.C., N.K.
Singh, P.M. Hasegawa and R.A. Bressan. 1989. Stable NaCl tolerance of
tobacco cells is associated with accumulation
of osmotin. Plant Physiol. 91:855-861.
Iraki, N.M., N.K.
Singh, R.A. Bressan and N.C. Carpita.
1989. Cell wall organization of
tobacco cell adapted to salt and draught stress. Plant Physiol. 91:48-53.
Singh,
N.K., D.E. Nelson, D.N. Kuhn, P.M. Hasegawa and R.A.
Bressan. 1989. Molecular cloning of osmotin
and regulation of its expression by
ABA and adaptation to low water potential.
Plant Physiol. 90:1096-1101.
Singh,
N.K., D.E. Nelson, P.C. LaRosa, P.M. Hasegawa and R.A.
Bressan. 1989. Osmotin: A protein
associated with osmotic stress adaptation
in plant cells. In J.H. Cherry,
ed. Environmental Stress in Plants:
Physiological and Biochemical Basis. Springer Verlag. pp. 67-87.
Singh,
N.K., P.C.
LaRosa, D.E. Nelson, N.M. Iraki, P.M. Hasegawa, N.C. Carpita, and R.A.
Bressan. 1989. Reduced growth rate and changes in cell wall of plant cells
adapted to NaCl. Ibid. pp. 173-194.
Singh,
N.K., C.A. Bracker, P.M. Hasegawa, A.K. Handa, S. Buckel,
M.A. Hermodson, E. Pfankoch, F.E. Regnier and R.A. Bressan. 1987. Characterization of osmotin: A
thaumatin like protein associated with osmotic adaptation in plant cells. Plant
Physiol. 85:529-536.
Bressan, R.A., N.K.
Singh, A.K. Handa, R. Mount, J. Clithero and P.M. Hasegawa. 1987.
Stability of altered genetic expression in cultured
plant cells adapted to salt. In L. Monti, ed. Draught Resistance in
Plants: Genetic and Physiological Aspects. Comm. Europ. Commun. Luxenborg. Pp. 41-58.
Singh,
N.K., P.C.
LaRosa, P.M. Hasegawa, A.K. Handa and R.A. Bressan. 1986. Hormonal regulation of protein synthesis associated with NaCl adaptation in
plant cells. Proc. Natl. Acad. Sci. U.S.A. 84:739-743.