Narendra K. Singh

Office: 326 Life Sciences Building
Laboratory: 334 Life Sciences Building
Phone: (334) 844-1667/ 1645 (FAX) 

Ph.D. Molecular Biology (1979) - Tata Institute of Fundamental Research, Mumbai, India
M.S. Botany (1967) - Patna University, India                                   
B.S. Honors (1964) - Patna University, India                

Professor (1995 - present) – Auburn University, Alabama    
Associate Professor (1989 - 1995) – Auburn University, Alabama
Associate Professor (1988 - 1989) – Virginia Commonwealth University, Virginia   
Research Associate (1982 - 1987) – Purdue University, Indiana
Lecturer and Reader (1968 - 1982) – Ranchi University, India 

Courses taught at Auburn University

  • Principles of Biology (BIOL 1020)
  • Honors Biology (BIOL 1027)
  • Plant Biology (BIOL 3200)
  • Plant Gene Expression (BIOL 5320/6320)
  • Introductory Molecular Genetics (BIOL 5220/6220)
  • Recombinant DNA and Gene Expression (BIOL 5521/6521)
  • Undergraduate seminars (BIOL 4950): Protein sorting, Transgenics, Central dogma exceptions, Small regulatory RNA
  • Special topics (BIOL 7970): Stress proteins, Molecular biology of plant development
  • Undergraduate research (BIOL 4980)
  • Special problems (BIOL 7980)

Major professor of graduate students and their current affiliations

  • Yuying Sang, Ph.D. 2008: Assistant Professor, Academy of Sciences, Shanghai Institute of Plant Physiology and Environment, China
  • Nisreen Al-Quraan, Ph.D. 2008: Assistant Professor, Department of Biotechnology & Genetic Engineering, Jordan University of Science & Technology, Jordan
  • Kun Yuan, Ph.D. 2008: Assistant Professor, South China University of Science & Technology, Guandong, China (Co-advisor)
  • Janani Shutthanandan, M.S.2002: Research Associate, Pacific Northwest National Lab., Richland, WA
  • Quing Luo, M.S. 2000: Present position not known
  • Shu-Yi Wang, Ph.D. 2000: Present position not known
  • Sagadevan Mundree, Ph.D. 1996: Professor & Deputy Director, Center for Tropical Crops, Queensland University of Technology, Australia
  • Roland W. Herzog, Ph.D. 1996: Professor, Division of Cellular & Molecular Therapy, Department of Pediatrics, University of Florida, FL
  • Rajendra J. Redkar, Ph.D. 1995: Senior Scientist, Mol. Biol., Schott Glass Inc., Dureya, PA
  • Ching-Chun Chang, M.S. 1994: Associate Professor, Plant Sciences, National Cheng Kung University, Taiwan

Current research interest

            ●    Responses to environmental stress: changes in gene expression profile in response to heat and draught  

            ●    Edible vaccines: development of plant derived edible vaccines against viral diseases


Publications (1986-2011)


  • Pant, S.R., N.K. Singh, D. Weaver, C-C. Weng, M. Zhong, and R.D. Locy. 2011. Changes in gene expression associated with heat stress in cotton as determined by deep sequencing of cDNA libraries. Proc. Beltwide Cotton Conf. (In Press).
  • Hu, H,  N.K. Singh, D. Weaver, Pant, S.R., M. Egnin, M. Zhong, C-C. Weng, and R.D. Locy. 2011. Characterization of cotton leaf miRNA and changes in miRNA expression associated with heat stress in cotton as determined by deep sequencing. Proc. Beltwide. Cooton. Conf. (In press).
  • Al-Quaraan, N.A., R.D. Locy, and N.K. Singh. 2011. Implication of paraquat and hydrogen peroxide-induced oxidative stress treatments on the GABA shunt pathway in Arabidopsis thaliana calmodulin mutants. Plant Biotech Rep. 5: 225-234.
  • Wu, H-Z, K. S. Gunn, S.R. Singh,  N.K. Singh, S-C Ou, and J.J. Giambrone. 2011. DNA vaccine against H1N1 avian influenza virus-induced humoral and cell-mediated immunity in SPF chickens. Jour Biotech Res. 3:27-36.
  • Wu, H-Z, K. S. Gunn,  N.K. Singh, S-C Ou, and J.J. Giambrone. 2011. Yeast-derived avian influenza virus hemagglutin protein induced immune response in SPF chicken. J. Animal Vet Advances. 10(8): 999-1004.
  • Tzou, Y-M., T-S. Huang, B.A. Chin and N.K. Singh. 2011. Expression of tobacco Osmotin in Escherichia coli for biocidal activity. Biotechnology Letters. 33:539-543.
  • Sang, Y., R.D. Locy, L. Goertzen, Y-M, Tzou, K.Y. Kang and N.K. Singh. 2011. Identification of a second pridoxamine phosphate oxidase in Arabidopsis thaliana. Acta  Physiol. Plant. 33:559-567.
  • Sang, Y., L. Goertzen, A. Rashotte, Y. Si, R.D. Locy, and N.K. Singh. 2010. Expression, in vivo localization and phylogentic analysis of Arabidopsis Pyridoxine 5’-phosphate Oxidase. Plant Physiol. Biochem. 49:88-96.
  • Al-Quaraan, N.A., R.D. Locy, and N.K. Singh. 2010. Expression of Calmodulin genes in wild type and Calmodulin mutants of Arabidopsis thaliana under heat stress. Plant Phyiol. Biochem. 48:697-703.
  • Ying, S., F. Dane, A. Rashotte, K. Kang, and N.K. Singh. 2010. Cloning and expression analysis of Ccrboh gene encoding respiratory burst oxidase in Citrullus colocanthus and grafting onto Citrullus lanatus (watermelon). J. Experimental Bot. 61:1635-1642.
  • Barbosa, J.M., N.K. Singh, J.H. Cherry and R.D. Locy. 2010. Nitrate uptake and utilization is modulated by exogenous γ-aminobutyric acid in Arabidopsis thaliana seedlings. Plant Physiol. Biochem. 48:443-450.
  • Wu, H-Z., K. Wiiliams, S.R. Singh, K.S. Gunn, N.K. Singh, D. Teressa, and J.J. Giambrone. 2009. Integrity of recombinant hemagglutinin protein of Avian Influenza Virus H10N7. Biotechnology letters. 31: 1511-1517.
  • Wu, H-Z., K.S. Gunn, N.K. Singh and J.J. Giambrone. 2009. Development of plant based vaccine against reovirus. Avian Diseases. 53: 376-381. 
  • Parkhi,V., V.Kumar, G.Sunilkumar, L.M.Campbell, N.K.Singh and K.S.Rathore. 2009. Expression of apoplastically-secreted tobacco osmotin in cotton confers drought tolerance. Mol Breeding. 23:625-639.
  • Giambrone, J.J., T. Dormitorio, H-Z. Wu, N.K. Singh. 2008. Development of a recombinant avian influenza vaccine in yeast. Feedinfo. January, 2008.
  • Sang, Y., J. M. Barbosa, H-Z, Wu, R.D. Locy and N.K. Singh. 2007. Identification of pyridoxine (pyridoxamine) 5’ phosphate oxidase from Arabidopsis thaliana. FEBS Letters. 581: 344-349
  • Wu, H-Z., K.S. Gunn, N.K. Singh, R.D. Locy, and J.J. Giambrone. 2005. Yeast derived sigma C protein induced immunity against avian reovirus. Avian Disease. 49:281-285.
  • Zhang, C., L. Ping, F. Dane, R. Ebel, N.K. Singh, R.D. Locy, and W. Dozier. 2005. Cold acclimation induced genes in trifoliate orange, Poncirus trifoliate. Plant Cell Reporter 23:764-769.
  • Zhang, C., L. Ping, F. Dane, R. Ebel, N.K. Singh, R.D. Locy, and W. Dozier. 2005. Down regulated gene expression of cold acclimated Poncirus trifoliate. Can. J. Plant. Sci. 85: 417-424.
  • Wu, H-Z., N.K. Singh, R.D. Locy, K.S. Gunn, and J.J. Giambrone. 2004. Immunization of chicken with VP2 protein of infectious bursal disease virus expressed in Arabidopsis thaliana. Avian Disease 48:663-668.
  • Wu, H-Z., N.K. Singh, R.D. Locy, K.S. Gunn, and J.J. Giambrone. 2004. Comparison of avian reovirus S1 genes. Jour Anim. Vet. Adv.3: 326-329.
  • Wu, H., N.K. Singh, R.D. Locy, K-S. Gunn, and J.J. Giambrone. 2004. Orally fed transgenic plants containing VP2 of IBDV immunizes chickens. 5: 1-5.
  • Wu, H-Z, N.K. Singh, R.D. Locy, K.S. Gunn, and J.J. Giambrone. 2004. Expression of immunogenic VP2 of IBDV in transgenic Arabidopsis thaliana. Biotechnology letters 26: 787-792.
  • Lugo, S.K., M. Kunnimalayaan, N.K. Singh, and B.L. Nielsen. 2004. Required sequence elements for chloroplast DNA replication activity in vitro and in electroporated chloroplast. Plant Sci. 166: 151-161.
  • Giambrone, J.J., N.K. Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2003. Development of edible transgenic plant vaccine for immunizing poultry. Current Concept in Production. Pp. 1-3.
  • Narsimhan, M.L., H. Lee, B. Damsz, N.K. Singh, J.I. Ibeas, T.K. Mastmuto, C.P. Woloshuk, and R.A. Bressan.2003. Overexpression of a cell wall glycoprotein in Fusarium oxysporum increases virulence and resistance to a plant PR-5 protein. Plant Jour. 36: 390-400.
  • Giambrone, J.J., N.K. Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2002. Edible inoculations: developing edible transgenic plant vaccine to immunize chicken against IBDV. AAES Highlights. 9: 1-3.
  • Giambrone, J.J., N.K. Singh, R.D. Locy, K.S. Gunn and H-Z. Wu. 2001. Development of edible transgenic plant vaccine for immunizing chicken against IBDV. 3: 1-3.
  • Giambrone, J.J., N.K. Singh, R.D. Locy, K. Scissum-Gunn, and H. Wu. 2001. Transgenic plant vaccine to immunize chicken against infectious Bursal disease virus. Highlights in Agriculture. 48 (4).
  • Wang, S-Y, S-J. Wu, G. Thottappilly, R.D. Locy, and N.K. Singh. 2001. Molecular cloning and structural analysis of the gene encoding Bacillus cereus exochitinase Chi36. JBB 92:59-66.
  •  Wang, S-Y, A-L. Moyne, G. Thottappilly, S-J. Wu, R.D. Locy, and N.K. Singh. 2001. Purification and characterization of a Bacillus cereus exochitinase. Enzyme Microbial Technol. 28:492-498.
  • Wu, S-J., R.D. Locy, J.J. Shaw, J.H. Cherry and N.K. Singh. 2000. Mutation in Arabidopsis HIT1 locus causing heat and osmotic hypersensitivity. J. Plant Physiol. 157:543-548.
  • Singh, N.K., S.G. Mundree, and R.D. Locy. 2000. Novel determinants of salinity tolerance. In: Plant Tolerance to Abiotic Stresses in Agriculture: Role of Genetic Engineering (eds. J.H. Cherry) Kluver Publication. pp 131-138.
  • Barbosa, J.M., R.D. Locy, T.W. Barger, N.K. Singh and J.H. Cherry. 2000. GABA increases the rate of nitrate uptake and utilization in Arabidopsis roots. Ibid. pp. 53-63.
  • Locy, R.D., S-J. Wu, J. Bistnette, T.W. Barger, D. McNabb, M. Zik, H. Fromm, M.R. Knight, N.K. Singh and J.H. Cherry. 2000. The regulation of GABA accumulation by heat stress-induced increases in cytoplasmic calcium. Ibid. p. 39-52.
  • Thottappilly, G., H.D. Mingnouna, A. Onasanya, M. Abang, O. Oyelakin, and N.K. Singh. 1999. Identification and differentiation of isolates of Colletotrichum gleosporoides from Yam by random amplified polymorphic DNA markers. African Crop Science Journal. 7: 195-205.
  • Singh, N.K. 1999. Genetic transformation of edible fungi. Proc. International Mushroom Conference. 49-53.
  • Redkar, R.J., R.W. Herzog and N.K. Singh. 1998. Transcriptional activation of the Aspergillus nidulans gpd promoter by osmotic signals. Applied Env. Microbiol. 64:2229-2234.
  • Herzog. R.W., N.K. Singh, D.W. Urry and H. Daniell. 1997.  Expression of synthetic protein based polymer (Elastomer) gene in Aspergillus nidulans. Applied Microbiol. and Biotech. 47:368-372.
  • Johnson, B.S., N.K. Singh, J.H. Cherry, and R.D. Locy. 1997.  Purification and characterization of cowpea glutamate decarboxcylase. Phytochem. 46:39-44.
  • Chang, C.C., R.D. Locy, R. Smeda, S.V. Sahi and N.K. Singh. 1997. Photoautotrophic tobacco cells adapted to grow in high salinity. Plant Cell Report. 16:495-502.
  • Chang, P.F., B. Damsz, A.K. Kononowicz, A. Chen, Yi Xu, N.K. Singh, M.L. Narshimhan,  P.M. Hasegawa, and R.A. Bressan. 1996. Alteration in cell membrane associated protein after adaptation to osmotic stress. Physiol. Plant. 98:505-516.
  • Abed, L.R., D. Liu, M.P. Durzo, M.L. Narsimhan, N.K. Singh, P.M. Hasegawa and. R.A. Bressan. 1996. The antifungal activity of osmotin involves the fungal plasma membrane and cell wall. Plant Science. 118:11-23.
  • Chen, L., C.M. Peterson, and N.K. Singh. 1996. Isolation of cDNA clones from petiole abscission zones of cotton. Proc. Belt. Cotton Con. 2:1243-1246.
  • Redkar, R.J., P.A. Lemke and N.K. Singh.  1996. Altered gene expression in Aspergillus  nidulans in response to salt stress. Mycologia 88:256-263.
  • Herzog, R.W., N.K. Singh, H. Daniell and P.A. Lemke.  1996. A comparative study on transformation of Aspergillus nidulans by microprojectile bombardment of conidia and protoplast treated with polyethylene glycol.  Applied Microbiol. Biotech 45:333-339.
  • Locy, R.D., C.C. Chang, B.L. Nielsen and N.K. Singh. 1996. Photosynthesis in salt-adapted heterotrophic tobacco cells and regenerated plants. Plant Physiol. 110:321-328.
  • Redkar, R.J., P.A. Lemke and N.K. Singh. 1996. Isolation of differentially expressed cDNA clones from salt- adapted Aspergillus nidulans. Current Genetics. 29:130-135.
  • Redkar, R.J., R.D. Locy and N.K. Singh. 1995. Biosynthetic pathways of glycerol accumulation under salt stress in Aspergillus nidulans. Experimental Mycology. 19:241-246.
  • Herzog, R.W., N.K. Singh, C. Schmidt and P.A. Lemke. 1995. Presence of a P1 bacteriophage sequence in transforming plasmids of Pleurotus ostreatus. Current Genetics. 27:460-465.
  • Lemke, P.A., N.K. Singh and U-A Temann.  1994.  Genetic transformation of Ectomycorrhizal Fungi.  In: Mycorrhiza: Structure, Function, Molecular Biology and Biotechnology (eds.A. Varma and B. Hock). Springer-Verlag. pp. 137-156.
  • Kononowicz, A.K., K.G. Raghothama, A.M. Casas, D.E. Nelson, D. Lieu, M.L. Narsimhan, P.C. LaRosa, N.K. Singh, R.A. Bressan and P.M. Hasegawa. 1994. Structure, regulation and function of osmotin gene. In: Biochemical and Cellular Mechanisms of Stress Tolerance in Plants (ed. J.H. Cherry) Springer-Verlag 381-414.
  • Cherry, J.H., R.M. Mayer, K. Heuss-LaRosa, P.M. Reddy and N.K. Singh.  1994. Altered gene expression in thermoadapted cultured cells of cowpea.  Ibid. 229-242.
  • Daniell, H., C. Guda, N.K. Singh, J.D. Weete, J.H. Cherry. 1994.  Photosynthesis, epiculticular wax and lipid changes in cowpea cultivars grown under hyperthermic conditions. Ibid. 213-227.
  • Smeda, R., P.M. Hasegawa, P.B. Goldsborough, N.K.Singh and S.C. Weller.  1993.  A serine to thrionine substitution in triazine herbicide-binding protein in potato cells results in atrazine resistance without impairing productivity. Plant Physiol. 103:911-917.
  • Peng, M., P.A. Lemke and N.K. Singh 1993.  A nucleotide sequence involved in replicative transformation of a filamentous fungus.  Curr. Genet. 24:114-121. 
  • Singh, N.K. and J.J. Shaw.  1992.  Physical methods of gene transfer in plants. In Biotechnology: Enhancing Research on Tropical Crops in Africa. (Eds. G. Thottapilly, L. Monti, M. Raj and A. Moore). pp. 203-210.
  • LaRosa, P.C., Z. Chen, D.E. Nelson, N.K. Singh, P.M. Hasegawa and R.A. Bressan.  1992. Osmotin gene regulation is post-transcriptionally regulated.  Plant Physiol. 100:409-415.
  • Peng, M., N.K. Singh and P.A. Lemke.  1992.  Recovery of recombinant plasmids from Pleurotus ostreatus transformants. Curr. Genet. 22:53-59.
  • Lemke, P.A., N.K. Singh, M. Peng.  1992.  Genetic Engineering of an Edible Mushroom Species. AAES Highlights. 39:16
  • Nelson, D.E., K.G. Raghothama, N.K. Singh, P.M. Hasegawa and R.A. Bressan.  1992.  Analysis of structure and transcriptional activation of the osmotin gene. Plant Molec. Biol. 19:577-588.
  • Kononowicz, A.K., D.E. Nelson, N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1992. Regulation of the Osmotin gene promoter. Plant Cell 4:513-524.
  • Casas, A.M., D.E. Nelson, K.G. Raghothama, M.P. Durzo, N.K. Singh, R.A. Bressan and P.M. Hasegawa. 1992.  Expression of OSM like genes in the halophyte Atriplex nummularia L. Plant Physiol. 99:329-337.
  • Charles, D.J., N.K. Singh and J.E. Simon.  1992.  The essential oils of Alpinia galanga wild. Journ. Essential Oil Res. 4:81-83.
  • Narsimhan, M.C., M.L. Binzel, E. Perez-Prat, Z. Chen, D.E. Nelson, N.K. Singh, R.A. Bressan and P.M. Hasegawa.  1991. NaCl regulation of the gene encoding the 70 kDa subunit of tonoplast ATPase in tobacco cells. Plant Physiol. 97:562-568.
  • Dylweski, D.P., N.K. Singh and J.H. Cherry.  1991. Effects of heat shock and thermo-adaptation the ultrastructure of Cowpea (Vigna unguiculata) cells. Protoplasma. 163:125-135.
  • Pratt, R.C., N.K. Singh, R.E. Shade and R.A. Bressan.  1990.  Purification and characteri-zation of isolectins from tepary bean (P. acutifolius) which delay Bruchid  Beetle development. Plant Physiol. 93:1453-1459.
  • Bressan, R.A., D.E. Nelson, N.M. Iraki, P.C. LaRosa, N.K. Singh, P.M. Hasegawa and N.C. Carpita.  1990. Reduced cell expansion and changes in cell walls of plant cells adaptedto NaCl.  In Environmental Injury to Plants.  F. Katterman (ed.) Academic Press. pp, 137-171.
  • Carpita, N.C., N.K. Singh, R.A. Bressan, P.M. Hasegawa, S.R. Schnapp. 1990. Cellular mechanisms of salt and water stress tolerance in plants.  Acta Horticulturae. 280:341-352.
  • LaRosa, P.C., N.K. Singh, P.M. Hasegawa and R.A. Bressan. 1989. Stable NaCl tolerance of tobacco cells is associated with accumulation of osmotin. Plant Physiol. 91:855-861.            
  • Iraki, N.M., N.K. Singh, R.A. Bressan and N.C. Carpita.  1989.  Cell wall organization of tobacco cell adapted to salt and draught stress.  Plant Physiol. 91:48-53.
  • Singh, N.K., D.E. Nelson, D.N. Kuhn, P.M. Hasegawa and R.A. Bressan.  1989. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential.  Plant  Physiol. 90:1096-1101.
  • Singh, N.K., D.E. Nelson, P.C. LaRosa, P.M. Hasegawa and R.A. Bressan.  1989. Osmotin: A protein associated with osmotic stress adaptation in plant cells.  In J.H. Cherry, ed. Environmental Stress  in Plants: Physiological and Biochemical Basis. Springer Verlag.       pp. 67-87.
  • Singh, N.K., P.C. LaRosa, D.E. Nelson, N.M. Iraki, P.M. Hasegawa, N.C. Carpita, and R.A. Bressan.  1989. Reduced growth rate and changes in cell wall of plant cells adapted to NaCl.  Ibid. pp. 173-194.
  • Singh, N.K., C.A. Bracker, P.M. Hasegawa, A.K. Handa, S. Buckel, M.A. Hermodson, E. Pfankoch, F.E. Regnier and R.A. Bressan. 1987. Characterization of osmotin: A thaumatin like protein associated with osmotic adaptation in plant cells. Plant Physiol. 85:529-536.
  • Bressan, R.A., N.K. Singh, A.K. Handa, R. Mount, J. Clithero and P.M. Hasegawa. 1987. Stability of altered genetic expression in cultured plant cells adapted to salt. In L. Monti, ed. Draught Resistance in Plants: Genetic and Physiological Aspects. Comm. Europ. Commun. Luxenborg. Pp. 41-58.
  • Singh, N.K., P.C. LaRosa, P.M. Hasegawa, A.K. Handa and R.A. Bressan.  1986. Hormonal regulation of protein synthesis associated with NaCl adaptation in plant cells. Proc. Natl. Acad. Sci. U.S.A. 84:739-743.