COSAM » Departments » Chemistry & Biochemistry » Facilities » Mass Spec Center

Mass Spectrometry Center

MSC provides collaborative assistance to PIs in collection of preliminary data, preparation of proposals and publication of manuscripts. Our contributions involve consultation, data analysis, and the preparation and review of manuscripts. We provide letters of support to PIs that describe the instrumentation and capabilities of the MSC.

Types of Analysis we provide include:

  • Small molecules  
  • Large molecules especially proteins 
    • Whole protein analysis with ESI and calculation of the protein mass through deconvolution
    • Digestion with protease(s) and separation via LC and MS/MS to determine peptide sequence usually leading to protein identity with MASCOT (also known as protein sequencing, shotgun proteomics, or bottoms-up)
  • Nucleotides
    • Small DNA and RNA sequences can be analyzed with ESI and the molecule mass calculated with deconvolution
    • Sample desalting is usually necessary

UPLC

LC/MS (Waters® Acquity UPLC™ and Q-Tof Premier™) UPLC is used for separation of molecules at ultra high pressure. Q-Tof Premier Mass Spectrometer is a hybrid quadrupole, orthogonal acceleration time-of-flight mass spectrometer. UPLC/Q-Tof enables automated exact mass measurement of precursor and fragment ions.

GCT Premier

GC/MS (Waters GCT Premier)The GCT Premier™ is a bench top orthogonal acceleration time-of-flight (oa-TOF) mass spectrometer. GC/MS has a variety of inlet, ion source and software options for analysis of volatiles.

 

Mass Spectrometry Center
172 Chemistry Building
Melissa Boersma
mdb0067@auburn.edu
(334) 844-6911

Rates for federally funded projects are $40 per hour.



Last Updated: 06/27/2017