The Laboratories of Dr. Joseph Giambrone

Dr. Giambrone's current research is on viral diseases of young chickens. He is working on infectious bursal disease virus and reoviruses infections. These viruses cause common infections, which can result in morbidity, mortality, and/ or immunosuppression in young chickens. He has developed improved diagnostic techniques to detect the presence of these viruses in chickens using the latest molecular biological techniques such as monoclonal antibodies, recombinant probes, the polymerase chain reaction (PCR) test and sequencing of nucleic acids. Dr. Giambrone has used these reagents and procedures to develop immunoperoxidase assays, in situ hybridization assays, PCR restriction fragment length polymorphism tests, and in situ PCR hybridization tests. He has also used conventional virological techniques to develop and/or test the efficacy of vaccines against these important viral diseases of chickens.

1. Liu, Hung-Jen, J.J. Giambrone, and B.O. Nielsen, 1997. Molecular characterization of avian reoviruses using nested PCR and nucleotide sequence analysis. J. Virol. Meth. 65:159-167.
2. Liu, Hung-Jen and J.J. Giambrone, 1997. In situ detection of reovirus in formalin-fixed, paraffin-embedded chicken tissues using a digoxigenen-labeled cDNA probe. Avian Dis. 41:447-451.
3. Liu, Hung-Jen and J.J. Giambrone, 1997. Characterization of a nonradioactive cloned cDNA probe for detecting avian reoviruses. Avian Dis. 41:374-378.
4. Liu, X., J. J. Giambrone, and T. Dormitioro, 1998. Simplified sample processing combined with a sensitive nested polymerase chain reaction assay for detection of infectious bursal disease virus in the bursa of Fabricius. Avian Dis. 42: 480-485, 1998.
5. Chen, H. Y., Q. Zhou, M. F. Zhang, and J. J. Giambrone, 1998. Sequence analysis of the VP2 hypervariable region of nine infectious bursal disease virus isolates from Mainland China. Avian Dis. 42:762-769.
6. Giambrone, J. J., T. V. Dormitorio, T. Brown, and K. Takeshita, 1999. Monitoring of the immune status of broiler breeders against infectious bursal disease virus using progeny challenge and serological data. J. of Appl. Poultry Sci. 8:362-367.
7. Liu, X., J. J. Giambrone, and F. J. Hoerr, 2000. In situ hybridization, immunohistochemistry, and in situ reverse transcription-polymerase chain reaction for the detection of infectious bursal disease virus. Avian Dis. 44: 161-169.
8. Liu H., J. J. Giambrone, Y. H. Wu, M. H. Liao, and C. F. Lu, 2000. The use of monoclonal antibody probes for the detection of avian reovirus antigens. J. Virol. Meth. 86:1115-120.